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目的研究纳洛酮对人离体肾脏缺血再灌注损伤的影响。方法用人离体肾脏 ,实验组在灌注液中加入纳洛酮(NAL)0.8μg/ml,分别在0～10min、10～20min和20～30min时收集灌注流出液 ,测定其丙二醛(MDA)、过氧化物歧化酶(SOD)及肾组织匀浆中的Na+ ,K+ _ATP酶、Ca2 +_ATP酶含量 ,并做病理观察。结果上述各项指标在用NAL组与对照组比较均无显著性差异。结论NAL对离体肾脏缺血再灌注无明显影响 ,NAL对在体肾脏缺血再灌注的保护作用不是直接作用在肾脏本身。
Objective To investigate the effect of naloxone on renal ischemia-reperfusion injury in humans. Methods Renal kidneys were used in the experiment. Naloxone (NAL) 0.8μg / ml was added to the perfusion solution. Perfused effluent was collected at 0-10min, 10-20min and 20-30min, respectively. Malondialdehyde (MDA) ), Superoxide dismutase (SOD) and renal tissue homogenate Na +, K + _ATP enzyme, Ca2 + _ATP enzyme content, and pathological observation. Results The above indexes had no significant difference between NAL group and control group. Conclusion NAL has no obvious effect on isolated renal ischemia-reperfusion injury. The protective effect of NAL on renal ischemia-reperfusion injury is not directly related to the kidney itself.