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Objective: To investigate the possible enhancement of immuno-genicity of the carboxyl-terminal peptide of β-hCG which is made upof 37 amino acid residues (109145) and contains the specific epitope(antigenic determinant) of hCG. Materials & Methods: hCGβ-CTP37 tetraploid cDNA was constructed by linking four hCGβ-CTP37cDNAs together. The product was then subcloned into the E. coli ex-pression vector pQE60 to construct the expression vector pQE60/(hCGβ-CTP37)_4. Recombinant (hCGβ-CTP37)_4 was expressed in E.coli-X-blue. Results: Western blot analysis showed that the te-traploid of hCGβ-CTP37 had an apparent molecular weight of 20 kDand a relatively stronger anti-hCG antibody-binding activity comparedwith the diploid form. Conclusion: The tetraploid of hCGβ-CTP37may be a more potent immunogen for raising anti-hCG vaccines forfertility regulation or tumor suppression. (Reprod Contracep 2000; 11:113-119)
Objective: To investigate the possible enhancement of immuno-genicity of the carboxyl-terminal peptide of β-hCG which is made up of 37 amino acid residues (109145) and contains the specific epitope (antigenic determinant) of hCG. Materials & Methods: hCGβ- CTP37 tetraploid cDNA was constructed by linking four hCGβ-CTP37 cDNAs together. The product was then subcloned into the E. coli ex-pression vector pQE60 to construct the expression vector pQE60 / (hCGβ-CTP37) _4. Recombinant (hCGβ-CTP37) expressed in E. coli-X-blue. Results: Western blot analysis showed that the te-traploid of hCGβ-CTP37 had an apparent molecular weight of 20 kD and a relatively stronger anti-hCG antibody-binding activity compared to the diploid form. Conclusion: The tetraploid of hCGβ-CTP37may be a more potent immunogen for raising anti-hCG vaccines forfertility regulation or tumor suppression. (Reprod Contracep 2000; 11: 113-119)