目的提纯大鼠脑胶质瘤C6细胞热休克蛋白抗原肽复合物(HAC),免疫SD大鼠,观察HAC的抑瘤作用。方法采用免疫亲和层析方法提纯大鼠脑胶质瘤C6细胞HAC,免疫20只大鼠为实验组,以另20只大鼠作为对照组,于免疫后1 w,采用立体定向脑内接种方法,以C6细胞攻击两组大鼠,于肿瘤细胞攻击后第二周,取两组动物外周静脉血,测定外周静脉血淋巴细胞计数,并应用流式细胞仪技术测定外周血中CD3+/CD4+和CD3+/CD8+T淋巴细胞的比例。观察饲养过程中实验动物出现的症状、体征和第四周实验动物存活率。于第四周处死存活动物,取脑组织进行HE染色病理组织学检查,并用免疫组化方法分析脑胶质瘤浸润区T淋巴细胞分布情况。结果实验组大鼠外周血淋巴细胞计数显著高于对照组(P<0.01),CD3+/CD4+和CD3+/CD8+T淋巴细胞的比例实验组均显著高于对照组(P<0.01)。实验组动物症状出现时间显著晚于对照组动物(P<0.01),实验组动物四周末存活率显著高于对照组(P<0.05)。实验组胶质瘤局部浸润的CD3+和CD4+细胞数均显著高于对照组(P<0.01),实验组胶质瘤局部浸润的CD8+细胞数与对照组比较无显著差异(P>0.05),实验组胶质瘤局部浸润T淋巴细胞CD4+/CD8+显著高于对照组(P<0.01)。结论C6细胞中HAC可以诱导大鼠产生对C6细胞的细胞免疫,提高大鼠存活率。 Objective To purify the heat shock protein antigen peptide complex (HAC) of rat glioma C6 cells and immunostimulate SD rats to observe the antitumor effect of HAC. Methods Immunoaffinity chromatography was used to purify HAC from rat C6 glioma cells. Twenty rats were immunized with 20 rats as experimental group and another 20 rats as control group. After 1 w of immunization, Methods Two groups of rats were challenged with C6 cells. Peripheral blood was collected from the peripheral blood of two groups in the second week after the tumor cells were challenged. The peripheral blood lymphocyte counts were measured. Flow cytometry was used to determine the levels of CD3 + / CD4 + And CD3 + / CD8 + T lymphocyte ratio. Observe the symptoms, signs and experimental animals in the fourth week during the breeding process. Survival animals were killed in the fourth week, brain tissue was taken for histopathological examination by HE staining, and the distribution of T lymphocytes in glioma infiltration area was analyzed by immunohistochemistry. Results The number of peripheral blood lymphocytes in experimental group was significantly higher than that in control group (P <0.01). The ratio of CD3 + / CD4 + and CD3 + / CD8 + T lymphocytes in experimental group was significantly higher than that in control group (P <0.01). The symptoms of animals in the experimental group appeared significantly later than those in the control group (P <0.01), and the survival rate in the experimental group was significantly higher than that of the control group (P <0.05). The number of local infiltrated CD3 + and CD4 + cells in experimental group was significantly higher than that in control group (P <0.01). The number of local infiltrating CD8 + cells in experimental group was not significantly different from that in control group (P> 0.05) The level of CD4 + / CD8 + T lymphocytes in locally infiltrated glioma group was significantly higher than that in control group (P <0.01). Conclusion HAC in C6 cells can induce the cellular immunity of C6 cells in rats and improve the survival rate in rats.