The eggshell of Reeve’s pheasant (Syrmaticus reevesii) collected from the Dongzhai National Nature Reserve in Henan Province,China was studied.By using scanning electron microscopy and inductively coupled plasma (ICP) spectrometry,the ultrastructure and elemental composition of the eggshell was determined.The study showed that the average thickness of the surface layer of crystals,the palisade and cone layer,and the eggshell membrane were 20.8,220.8 and 62.5 l,respectively,accounting for 6.8%,72.6% and 20.6% of the total thickness of the eggshell.There were many vesicular holes in the palisade layer with an average diameter of 0.32±0.08 μm(n = 30).The function of these holes might be significant to air exchange.The shape of the eggshell pore on the surface layer of crystals is round or elliptical.The fracture surface of the pore is funnel-shaped.Some granules filled the upper part of the eggshell pores.The content of 21 elements in the eggshell of wild and captive Reeve’s pheasants was compared and presented.It indicated that among the elements that made up the eggshell of the wild pheasant,the content of Ca,Mg,P and S was much higher,ω＞ 1 mg/g,with ω (Ca) being higher than 40% of the eggshell.The contents of Na,Si,Sr,K and A1 were ω = 0.1-1 mg/g,while Fe,Zn,Pb,Mn,Cu,V and Ti had lower concentrations (ω = 1-100 μg/g).The ω ofNi,Cr,Co,Se,Cd were lower than 1 μg/g.The elemental composition in the eggshell of the captive Reeve’s pheasant kept in the Dongzhai National Natural Reserve was significantly different from that of the wild species,with a difference of over 20% on S,Cu,Fe,AI,Mn,Si,Sr,Se and Cr.The lower intake of Fe,Mn,Si and Sr on the one hand and the higher intake of S,Cu,Al and Cr on the other hand might be responsible for the low fertility of captive Reeve’s pheasants in the Dongzhai National Nature Reserve.In order to ensure that the pheasants are receiving the proper amount of nutrition and to improve their breeding success,the amount of certain elements in the food should be adjusted.