植物名称密花美登木(Maytenus Con-fertiflorus)。材料类别茎段、叶柄和叶片。培养条件(1)培养基有Ms、B_5、White三种,附加2、4-Dlms/L+KT0.2mg/L激素组合。(2)培养室温度25—30℃,自然漫射光。结果接种后5—10天,茎段在切口两端开始膨大。10—15天以后,切口两端韧皮部部位开始长出白色松散的愈伤组织。约20—30天,愈伤组织逐渐长大呈圆球状或小瘤状,结构紧密。叶柄的愈伤化过程和茎段比较,通常较茎晚,愈伤组织白色,呈致密的圆球状、疏松粉状或水浸状,有的叶柄表现为整段破裂, Plant name Maytenus Con-fertiflorus. Material category Stems, petioles and leaves. Culture conditions (1) medium Ms, B_5, White three, with 2,4-Dlms / L + KT0.2mg / L hormone combination. (2) Incubation room temperature 25-30 ℃, naturally diffuse light. Results 5-10 days after inoculation, the stem segments began to expand at both ends of the incision. After 10-15 days, the phloem at both ends of the incision began to grow white, loose callus. About 20-30 days, callus gradually grow into spherical or nodular, compact structure. Petiole callus and stem segments compared, usually late stem, callus white, dense spherical, loose powder or flooded, and some petiole showed the entire rupture,