论文部分内容阅读
胶原酶法分离猪卵巢卵子,热溶解制备透明带抗原。以此抗原免疫新西兰雌兔15只,免疫动物不孕期5个月以上。免疫动物交配后在卵巢上未观察到排卵点。在免疫动物动情期卵巢组织切片上只观察到极少量初级和次级滤泡,原始滤泡正常。用荧光间接测定法测定抗血清滴度变化情况,其峰值发生在第四次免疫后第10天,并维特一个月,其后下降,在不孕期维持在1:500以上。5个半月时1只免疫兔获得妊娠,其抗血清滴度为1:100。动物卵子在体外经抗血清处理后,透明带表层产生抗原-抗体复合沉淀层,抑制了胰酶和链霉蛋白酶对透明带的消化。研究了抗血清对人精卵体外结合的影响,人卵子在体外经抗血清处理后,与精子的结合完全被抑制。
Isolation of porcine ovarian eggs by collagenase method, and preparation of zona pellucida antigen by thermal dissolution. 15 New Zealand female rabbits were immunized with the antigen, and the infective animals were immunized for more than 5 months. Ovulation was not observed on ovulation after immunization of animals. Only a very small amount of primary and secondary follicles were observed on the ovarian tissue sections during the estrous period of immunized animals, and the original follicles were normal. Fluorescence indirect assay was used to determine the change of antiserum titers. The peak value occurred on the 10th day after the fourth immunization. The peak value of vitreous was 1 month, then decreased, and remained at 1: 500 during infertility. Five and a half months, one immunized rabbit was given a pregnancy with an antisera titer of 1: 100. Animal eggs treated with antisera in vitro, the surface of the zona pellucida produces antigen - antibody composite sedimentation layer, inhibit the trypsin and pronase digestion of the zona pellucida. The effect of antiserum on the in vitro binding of human sperm eggs was studied. The binding of human oocytes to sperm was completely inhibited after antisera were treated in vitro.