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在利用反转录－ＰＣＲ从人胎肝中获得编码人血小板生成素（ｈＴＰＯ）全长ｃＤ－ＮＡ的基础上，综合ＴＰＯ结构与功能的研究信息，在大肠杆菌中表达了成熟肽Ｎ端结构域．目的蛋白在菌体内以包涵体形式存在，表达量约占菌体总蛋白的３０％；包涵体经变性、复性、凝胶过滤、离子交换层析等步骤处理后，所得产物给Ｂａｂｌ／ｃ小鼠腹腔连续注射８ｄ，第９ｄ摘眼球采血，计数血小板的数量．结果表明，ＴＰＯＮ端结构域具有明显促进血小板生成的作用．
On the basis of obtaining the full-length cD-NA encoding human thrombopoietin (hTPO) from human fetal liver by reverse transcription-PCR and synthesizing the research information on the structure and function of TPO, the N-terminal structure of mature peptide is expressed in E. coli area. The target protein exists in the form of inclusion body in the cell body, and the expression amount is about 30% of the total bacterial protein. After the inclusion body has been denatured, refolded, gel-filtered, and ion-exchange chromatographically processed, Mice were injected intraperitoneally for 8 days, blood was collected on the 9th day, and the number of platelets was counted. The results showed that TPON terminal domain has a significant role in promoting platelet production.