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目的 探讨癌相关基因APC、p16 INK4A、p2 1WAF1和c myc等在结肠癌中的表达及其受甲基化的调控。 方法 培养结肠癌细胞SW 1116、Colo 32 0、HT2 9,分别用不同浓度的 5 aza dC干预细胞。提取细胞的RNA ,用RT PCR的方法检测p16 INK4A、p2 1WAF1、APC和c myc等多种基因的表达情况 ;同时以流式细胞仪分析SW1116和Colo 32 0的细胞周期。结果 (1)三种细胞在干预前有较弱的 p16 INK4A和APC的表达 ,而在用 5 aza dC干预后表达增强 ,且在不同的结肠癌细胞株 5 aza dC作用发挥最佳的时间与浓度不同。 (2 ) p2 1WAF1在干预前后均不表达 ,c myc在干预前后表达无明显变化。 结论 三株人结肠癌细胞中 ,5 aza dC均可诱导 p16 INK4A和APC的表达 ,但对 p2 1WAF1和c myc无明显影响。
Objective To investigate the expression of cancer related genes APC, p16 INK4A, p2 1WAF1 and c myc in colon cancer and their regulation by methylation. Methods Colon cancer cells SW1116, Colo32, HT2 9 were cultured and treated with different concentrations of 5 aza dC. The cell RNA was extracted and the expression of p16 INK4A, p2 1WAF1, APC and c myc were detected by RT-PCR. The cell cycle of SW1116 and Colo 32 were analyzed by flow cytometry. Results (1) The three kinds of cells had weak expressions of p16 INK4A and APC before intervention, but increased after intervention with 5-aza dC, and the best time and effect of 5-aza dC were observed in different colon cancer cell lines Different concentrations. (2) p2 1WAF1 was not expressed before and after intervention, cmyc expression before and after intervention no significant change. Conclusions 5 aza dC can induce the expression of p16 INK4A and APC in all three human colon cancer cells, but have no significant effect on p2 1WAF1 and c myc.