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目的探讨铜和土霉素复合暴露对玉米幼苗生长发育的影响。方法观察铜(Cu~(2+))和土霉素(OTC)单一暴露及Cu~(2+)+OTC复合暴露对其发芽、总根长、叶片抗氧化酶和丙二醛含量的影响。结果 OTC、Cu~(2+)单一暴露、Cu~(2+)+OTC复合暴露对玉米种子发芽率的影响无统计学意义(P>0.05)。与对照组相比,Cu~(2+)(20、30 mg/L)单一暴露组总根长在8 h显著减低,随着生长时间的延续,Cu~(2+)(20、30和40 mg/L)单一暴露组总根长在96 h明显减低,差异有统计学意义(P<0.05);与对照组相比,Cu~(2+)+OTC(30、40 mg/L)组处理8 h玉米幼苗根系总根长缩短,随着玉米幼苗的生长,在24 h~96 h Cu~(2+)+OTC(10~40 mg/L)复合暴露组玉米幼苗根系总根长缩短,差异有统计学意义(P<0.05)。Cu~(2+)+OTC复合暴露与Cu~(2+)、OTC单一暴露对玉米幼苗叶片中超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)的活力没有明显影响(P>0.05)。与对照组相比,40 mg/L Cu~(2+)单一暴露组MDA含量的升高,差异有统计学意义(P<0.05);而OTC单一暴露组玉米幼苗叶片中MDA含量无明显变化(P>0.05);Cu~(2+)+OTC(20~40 mg/L)复合暴露组8~120 h MDA含量增加,差异有统计学意义(P<0.05)。结论 Cu~(2+)单一暴露和Cu~(2+)+OTC复合暴露对玉米幼苗总根长有一定的抑制作用,且叶片组织发生氧化损伤。
Objective To investigate the effects of copper and oxytetracycline composite exposure on the growth and development of corn seedlings. Methods The effects of single exposure of copper (Cu 2+) and oxytetracycline (OTC) and Cu 2+ (2+) OTC on their germination, total root length, leaf antioxidant enzymes and malondialdehyde . Results OTC, single Cu 2+ exposure and Cu 2+ + OTC combined exposure had no significant effect on seed germination rate of maize (P> 0.05). Compared with the control group, the total root length of Cu ~ (2 +) (20,30 mg / L) single exposed group decreased significantly at 8 h. With the extension of growth time, the Cu ~ (2 + 40 mg / L), the total root length of single exposure group decreased significantly at 96 h (P <0.05). Compared with the control group, Cu 2+ + OTC (30,40 mg / L) The total root length of maize seedlings rooted shortened in 8 h after treatment. With the growth of maize seedlings, the total root length of roots in maize seedlings exposed to Cu ~ (2 +) + OTC (10 ~ 40 mg / L) Shortened, the difference was statistically significant (P <0.05). Effects of Cu ~ (2 +) + OTC Combined Exposure and Cu ~ (2 +) and OTC Exposures on Superoxide Dismutase (SOD), Peroxidase (POD) and Catalase (CAT) in Leaves of Maize Seedlings Vitality had no significant effect (P> 0.05). Compared with the control group, the content of MDA in the single exposed group of 40 mg / L Cu 2+ increased significantly (P <0.05), while there was no significant change in the MDA content of the single exposed OTC group (P> 0.05). The content of MDA increased 8 ~ 120 h after exposure to Cu 2+ + OTC (20-40 mg / L), with statistical significance (P <0.05). Conclusion The single exposure of Cu 2+ and Cu 2+ + OTC compound exposure could inhibit the total root length of maize seedlings and oxidative damage of leaf tissue.