A long-term (1982-2001) field experiment was conducted in a calcareous soil under wheat (Triticum aestivum L.)-wheat(Triticum aestivum L.)-maize (Zea mays L.) rotation system at Zhangye, Gansu Province, China to determine the effects oflong-term fertilization on crop yield, nutrients interactions, content and accumulation of nitrate-N in soil profiles. Twenty-four plots in a split-plot factorial with a combination of eight treatments (from nitrogen (N), phosphorus (P), potassium (K)and farmyard manure (M) applications) and 3 replications were selected. Main treatments were M and without M, and thesub-treatments were no-fertilizer (CK), N, NP and NPK. When P and K fertilizers were part of treatments, their ratio to Nwas 1N:0.22P:0.42K. All M, P and K fertilizers were applied as the basal dressing. The grain yield was harvested eachexperimental period and straw yield for the period from 1988 to 1997. After crop harvest in 2000, the soil was sampled fromthe 0-20, 20-60, 60-100, 100-140 and 140-180 cm depths to determine NO3--N content. Maize yield of CK in 2000 was only 28.2%of that in 1984, and wheat in 2001 was 25.7% of that observed in 1982. Average impact of fertilizers on grain yield decreasedin the order of N > M > P > K. Yield response to N and P fertilizers increased with progress of the experiment. The impact ofK fertilizer showed no increase in grain yield during the initial 6 years (1982-1987), moderate increase in the next 5 years(1988-1992), and considerable increase in the last 9 years (1993-2001). The straw yield trend was similar to grain yield.Accumulation and distribution of NO3--N in soil was significantly affected by annual fertilizations. Mineral fertilizers (NPand NPK) led to NO3--N accumulation in most subsoil layers, with major impact in the 20-140 cm depth. The combinationof mineral fertilizers and farmyard manure (MNP and MNPK) reduced soil NO3--N accumulation in comparison to mineralfertilizers. It can be argued that long-term fertilization significantly enhanced grain and straw yield in this rotation scheme.The findings of this research suggest that it is important to balance application of mineral fertilizers and farmyard manurein order to protect soil and underground water from potential NO3--N pollution while sustaining high productivity in theoasis agro-ecosystem. A long-term (1982-2001) field experiment was conducted in a calcareous soil under wheat (Triticum aestivum L.) -maize (Zea mays L.) rotation system at Zhangye, Gansu Province, China Twenty-four plots in a split-plot factorial with a combination of eight treatments (from nitrogen (N), phosphorus (P), potassium (K) and farmyard manure (M) applications) and 3 replications were selected. Main treatments were M and without M, and thesub-treatments were no-fertilizer (CK), N, NP and NPK. When P and K fertilizers were part of treatments, their ratio to Nwas 1N: 0.22P: 0.42K. All M, P and K fertilizers were applied as the basal dressing. The grain yield was harvested eachexperimental period and straw yield for the period from 1988 to 1997. After crop harvest in 2000, the soil was sampled from the 0-20, 20-60, 60-100, 100-140 an Maize yield of CK was 2000 was only 28.2% of that in 1984, and wheat in 2001 was 25.7% of that observed in 1982. Average impact of fertilizers on grain yield decreased in the order of N> M> P> K. Yield response to N and P fertilizers increased with progress of the experiment. The impact of K fertilizer showed no increase in grain yield during the initial 6 years (1982-1987), moderate increase in the next 5 years (1988-1992), and considerable increase in the last 9 years (1993-2001). The straw yield trend was similar to grain yield. ACCUMULATION AND DISTRIBUTION OF NO3 - N in soil was significantly affected by annual fertilizations. The combination of mineral fertilizers and farmyard manure (MNP and MNPK) reduced soil NO3 - N accumulation in comparison (NPand NPK) led to NO3 - N accumulation in most subsoil layers, with major impact in the 20-140 cm depth. to mineralfertilizers. It can be argued that long-term fertilization significantly enhanced grain and straw yield in this rotation scheme. The findings of this research suggest that it is important to balance application of mineral fertilizers and farmyard manurein order to protect soil and underground water from potential NO3 - N pollution while sustaining high productivity in theoasis agro-ecosystem.