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目的探讨NK及NKT细胞在矽尘致大鼠肺纤维化外周血中的动态变化及临床意义。方法无特定病原体级健康成年Wistar大鼠70只,随机分成两组,每组各35只,实验组采用非暴露式经气管染尘法制备矽肺动物模型,对照组注入等体积的生理氯化钠溶液,分别于染尘后第l、3、7、14、28天各处死7只,采集外周血,用流式细胞术检测大鼠外周血中NK(CD3-/CD56+)及NKT(CD3+/CD56+)细胞的百分率。结果 NK细胞在实验组外周血的频率从染尘后的第1天开始就有所上升,于染尘后的第14天达到高峰,明显高于对照组,差异均有统计学意义(P<0.05或P<0.01);NKT细胞在实验组外周血的频率从染尘后的第1天开始与对照组比较明显下降,差异有统计学意义(P<0.05);随后呈现上升趋势,并于第14天达到高峰,但仍低于对照组,差异均有统计学意义(P<0.05)。结论 NK及NKT细胞在矽尘所致的肺纤维化中起一定的作用,可以作为阐明矽肺的免疫机制的1项免疫指标。
Objective To investigate the dynamic changes and clinical significance of NK and NKT cells in the peripheral blood of silicotic induced pulmonary fibrosis in rats. Methods Seventy healthy Wistar rats without specific pathogen were randomly divided into two groups with 35 rats in each group. Animals in the experimental group were treated with non-exposure endotracheal tube to produce silicotic animal model. The control group was infused with equal volumes of physiological sodium chloride Solution were collected. Seven rabbits were sacrificed on the 1st, 3rd, 7th, 14th and 28th day after the infection. Peripheral blood was collected and the levels of NK (CD3- / CD56 +) and NKT (CD3 + CD56 +) cells. Results The frequency of NK cells in the peripheral blood of the experimental group increased from the first day after dying, reaching the peak on the 14th day after the dying, which was significantly higher than that of the control group (P < 0.05 or P <0.01). The frequency of NKT cells in the peripheral blood of the experimental group decreased significantly from the first day after the dying day compared with the control group (P <0.05), and then showed an upward trend Reached the peak on the 14th day, but still lower than the control group, the differences were statistically significant (P <0.05). Conclusions NK and NKT cells play a role in the pulmonary fibrosis induced by silica dust and may be used as an immune index to clarify the immunological mechanism of silicosis.