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作者报道在支原体肺炎感染中,循环免疫复合物(CIC)的可能致病作用。方法:从114例(住院58例、门诊56例,年龄1～43岁)肺炎支原体感染患者中采血清标本。诊断依据为结合临床有冷凝集素滴度增高,补体结合(CF)滴度≥1:64或抗体滴度(CF和ELISA法)增高≥4倍,并排除其它疾病。46例的抗体无明显增高、但有特异性IgM抗体(ELISA法),提示近期感染。13例健康人血清作对照,先测冷凝集素及CF后贮于-20℃,供嗣后作ELISA检测。将肺炎支原体的MAC株纯化,在1/10mg的肺炎支原体抗原中含5μg蛋白质/ml,将其置于每个小凹中作ELISA检测。对114例急性期支原体肺炎者和13例健康对照者的血清,用ELISA法
The authors report the possible pathogenic role of circulating immune complexes (CICs) in mycoplasma pneumonia infections. Methods: Serum samples were collected from 114 patients (58 inpatients, 56 outpatients and 1 to 43 years old) with Mycoplasma pneumoniae infection. The diagnosis is based on an increase of clinical cold agglutinin titers, a> 4-fold increase in complement fixation (CF) titers of 1: 64 or antibody titers (CF and ELISA), and exclusion of other diseases. 46 cases of antibodies was not significantly increased, but with specific IgM antibodies (ELISA method), suggesting that recent infections. Thirteen healthy human sera were used as controls. After cryogenic agglutinin and CF were first stored at -20 ° C for subsequent ELISA testing. The Mycoplasma pneumoniae MAC strain was purified, containing 5 μg of protein / ml in 1/10 mg of Mycoplasma pneumoniae antigen and placed in each well for ELISA assay. Serum samples from 114 patients with acute mycoplasma pneumonia and 13 healthy controls were analyzed by ELISA