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砂培条件下,以2年生烟富3/八棱海棠(乔化)、烟富3/M7(半矮化)和烟富3/M26/八棱海棠(矮化)为试材,采用C、N双标记示踪技术,研究供氮水平(0N、25%N和100%N,100%N为Hoagland完全营养液中的N量)对不同砧穗组合苹果叶片衰老及~(13)C、~(15)N分配利用的影响.结果表明:在秋梢停长期,在同一氮素胁迫(0N和25%N)下植株叶片的叶绿素、氮含量和光合速率均以烟富3/八棱海棠最大,其次是烟富3/M7,烟富3/M26/八棱海棠最小,而正常氮素处理(100%N)均以烟富3/M26/八棱海棠最大,其次是烟富3/M7,烟富3/八棱海棠最小;在同一氮素胁迫水平下叶片的SOD和CAT活性均为烟富3/八棱海棠>烟富3/M7>烟富3/M26/八棱海棠,而正常氮素水平均为烟富3/M26/八棱海棠>烟富3/M7>烟富3/八棱海棠;在同一氮素水平下,3种砧穗组合苹果植株根和叶片的~(15)N、~(13)C分配率存在显著差异,氮素胁迫处理植株根的~(15)N、~(13)C分配率最高,表现为烟富3/八棱海棠>烟富3/M7>烟富3/M26/八棱海棠,而正常氮素处理植株叶片的~(15)N、~(13)C分配率最高,表现为烟富3/M26/八棱海棠>烟富3/M7>烟富3/八棱海棠.在不同氮素水平下,3种砧穗组合苹果植株氮肥利用率存在显著差异,且均以烟富3/八棱海棠植株最大,分别为44.3%、37.5%和31.4%,其次是烟富3/M7,分别为38.8%、30.7%和26.6%,烟富3/M26/八棱海棠最小,分别为32.0%、27.2%和22.5%.
Under the condition of sand culture, two-year-old Yanfu 3 / Baili Begonia (Qiaohua), Yanfu 3 / M7 (semi-dwarf) and Yanfu 3 / M26 / , N double labeling tracing technique was used to study the effect of nitrogen supply (0N, 25% N and 100% N, 100% N on the amount of N in Hoagland Complete Nutrient Solution) The results showed that the leaf chlorophyll and nitrogen content and photosynthetic rate under the same nitrogen stress (0N and 25% N) were all increased by 3/8 Begonia maxima followed by Fuming 3 / M7 and Yumingfu 3 / M26 / Begonia, while the normal nitrogen treatment (100% N) was the largest with Fuyan 3 / M26 / Begonia, followed by Fuye 3 / M7 and Yanfu 3 / Begonia tuberosum. The activities of SOD and CAT in leaves at the same level of nitrogen stress were YF3 / Begonia> YF3 / M7> YF3 / M26 Begonia tuberosum, and the normal nitrogen levels were all YF3 / M26 / Begonia> YF3 / M7> YF3 / Begonia tuberosus. Under the same nitrogen level, three kinds of scion combinations of apple plant roots and leaves (15) N and ~ (13) C, there was a significant difference in the distribution rate of ~ (15) N and ~ (13) C in the root of nitrogen stress treatment , And showed the highest distribution of ~ (15) N and ~ (13) C in the leaves of normal nitrogen-treated plants compared with that of Yanfu 3 / Haifeng> Yanfu 3 / M7> Yanfu 3 / M26 / The results showed that there was a significant difference in nitrogen use efficiency among the three combinations of scion and apples under different nitrogen levels The plants of YF-3 / Balenghaiguan were the largest, accounting for 44.3%, 37.5% and 31.4% respectively, followed by Fuming 3 / M7 with 38.8%, 30.7% and 26.6% respectively, and the lowest in Yuming Fu 3 / M26 / , Respectively 32.0%, 27.2% and 22.5%.