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为明确核盘菌的遗传多样性,对采自内蒙古和黑龙江不同地区的44株核盘菌进行了菌丝融合群确定,并比较了不同菌丝融合群间菌丝生长速度、致病力、草酸和总酸产量的差异。结果表明:供试44个菌株分为25个融合群,其中有14个融合群仅由单一菌株组成,所占比例为56.0%。菌丝融合群内和菌丝融合群间菌丝生长速度、致病力、草酸和总酸产量都表现出显著差异(P<0.001),并与菌株的地理来源无关。相关分析表明核盘菌菌株的致病力与菌株草酸产量呈正相关(r=0.484,P≤0.01),与pH呈负相关(r=-0.580,P≤0.01),与菌株的生长速度无关;草酸产量与pH高低(表示总酸的分泌量)负相关(r=-0.392,P≤0.01),进一步表明核盘菌菌株产生的总酸中草酸量占了很大的比例。
In order to clarify the genetic diversity of S. sclerotiorum, 44 strains of Sclerotinia sclerotiorum collected from different regions of Inner Mongolia and Heilongjiang were identified as mycelial fusion, and the mycelial growth rate, pathogenicity, Differences in oxalic acid and total acid yields. The results showed that 44 strains were divided into 25 groups, of which 14 were only a single strain, accounting for 56.0%. The mycelial growth rate, pathogenicity, oxalic acid and total acid production showed significant differences (P <0.001) between the mycelial fusion population and mycelial fusion population, and had no relation with the geographical origin of the strains. Correlation analysis showed that the pathogenicity of S. sclerotiorum was positively correlated with the yield of oxalate (r = 0.484, P≤0.01), negatively correlated with pH (r = -0.580, P≤0.01) The oxalate yield was negatively correlated with the level of pH (indicating the total acid secretion) (r = -0.392, P≤0.01), further indicating that the amount of oxalic acid produced by S. sclerotiorum accounts for a large proportion.