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BR D96N是具有显著光致变色特性的细菌视紫红质 (BR)经过基因定点突变的产物 ,与野生BR相比 ,它的M态寿命延长到了 5min ,因而表现出明显的饱和吸收特性和较低的饱和吸收光强 (0 .4mW /cm2 )。BR D96N用于全息记录时形成的是动态光栅 ,其特征量为光强而非曝光能量。实验表明 ,当再现光较弱时 ,衍射效率最大的记录光强基本对应于样品的饱和吸收光强。当记录光强一定时 ,再现光对所记录光栅具有擦除作用 ,再现光较弱时具有较高衍射效率 (1.8% ) ,但并不能得到高衍射光强 ,若要得到高衍射光强 ,存在着最佳再现光强 (80 μW /cm2 )。实验证明了在BR D96N薄膜上可以实现全息图像记录。
BR D96N is a product of site-directed mutagenesis of bacterial rhodopsin (BR) with significant photochromic properties and its M-state lifetime is extended to 5 min compared to wild-type BR, thus showing significant saturation absorption characteristics and lower The saturated absorption intensity (0.4 mW / cm2). The BR D96N is used for holographic recording to form dynamic rasters, which are characterized by light intensity rather than exposure energy. Experiments show that, when the reproduction light is weak, the diffraction efficiency of the largest recording light intensity corresponds to the saturation absorption intensity of the sample. When the recording light intensity is constant, the reproducing light has an erasing effect on the recorded grating. When the reproducing light is weaker, it has a higher diffraction efficiency (1.8%), but can not obtain a high diffraction intensity. To obtain a high diffraction intensity, There is an optimum reproducing light intensity (80 μW / cm 2). Experiments show that holographic image recording can be realized on the BR D96N film.