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目的建立快速、简便、能反映样品整体毒性的鱼贝类毒素筛查方法。方法以神经母细胞瘤细胞为实验材料,采用电压敏感荧光染料bis-oxonol,根据膝沟藻毒素2,3(GTX2,3)、短裸甲藻毒素(BTX)和河豚鱼毒(TTX)的毒理特点,观察不同浓度毒素作用下细胞荧光的变化。结果 GTX2,3的浓度在2～200ng/ml范围内,TTX的浓度在20～600ng/ml范围内,BTX的浓度在15～400ng/ml范围内,培养体系荧光强度与毒素的浓度之间存在很好的线性关系。结论利用电压敏感荧光染料法可实现对GTX2,3、TTX和BTX的快速检测。
Objective To establish a fast and simple method of screening fish and shellfish toxins that can reflect the overall toxicity of samples. Methods Using neuroblastoma cells as the experimental material, the voltage-sensitive fluorescent dye bis-oxonol was used to detect the apoptosis of neuroblastoma cells in vitro. According to the expression of GTX2,3, BTX and TTX Toxicological characteristics observed under different concentrations of toxin changes in cell fluorescence. Results The concentration of GTX2,3 in the range of 2 ~ 200ng / ml, the concentration of TTX in the range of 20 ~ 600ng / ml, the concentration of BTX in the range of 15 ~ 400ng / ml, the fluorescence intensity of the culture system and the concentration of toxins Very good linear relationship. Conclusion The rapid detection of GTX2, 3, TTX and BTX can be achieved by the voltage-sensitive fluorochrome method.