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背景:川芎嗪可抑制脑海马神经元的放电活动,在体内吸收后能有效地透过血脑屏障,并广泛分布于大脑皮质、脑干、纹状体、海马、小脑和中脑等部位。目的:探讨川芎嗪及其不同药物浓度经腹腔注射对青霉素致痫大鼠大脑皮质神经细胞结构的影响。设计:随机对照实验。单位:咸宁学院医学院生理学教研室。材料:实验于2004-09/2005-03在华中科技大学同济医学院解剖学教研室完成。选择健康清洁级SD 大鼠40只,雌雄不拘,体质量200～250g。随机分为5组,即手术对照组、青霉素致痫组和川芎嗪10m g/kg,20m g/kg,40m g/kg 组,每组8只。方法:大鼠麻醉开颅,暴露大脑皮质记录区域,采用BL-410生物功能实验系统记录左右两侧脑电,应用青霉素诱发青霉素致痫组和川芎嗪10m g/kg,20m g/kg,40m g/kg 组大鼠大脑皮质癫痫样放电。手术对照组在麻醉开颅手术1h 后取大脑;青霉素致痫组在青霉素诱发癫痫1h后取大脑;川芎嗪10m g/kg,20m g/kg,40m g/kg 组在青霉素诱发癫痫放电稳定后,再分别腹腔注射川芎嗪10m g/kg,20m g/kg,40m g/kg,待抑制作用最明显时取大脑。分别制备脑组织切片,苏木精-伊红染色,光学显微镜下观察。主要观察指标:各组大鼠大脑皮质神经细胞的结构变化。结果:40只大鼠全部进入结果分析,无脱失。①手术对照组大鼠大脑皮质神经细胞的形态结构正常。②青霉素致痫组大鼠大脑皮质神经细胞结构出现明显的改变,核固缩,胞浆溶解,出现空泡状结构,胞质内未见尼氏体。③川芎嗪10m g/kg 组大鼠与手术对照组比较,核固缩,胞浆溶解,出现空泡状结构,胞质内尼氏体减少。④川芎嗪20m g/kg 组大鼠与手术对照组比较,神经细胞内空泡减少,胞浆增多,胞浆内可见少量尼氏体。细胞结构形态有所改善。⑤川芎嗪40m g/kg 组大鼠与手术对照组比较,细胞核大而圆染色浅,胞质内可见块状的尼氏体,细胞结构形态趋于正常。结论:青霉素致痫后大鼠大脑皮质神经细胞的形态结构异常,不同剂量的川芎嗪可以不同程度地改善大脑皮质神经细胞的形态结构,而高剂量川芎嗪的改善作用尤其显著,其抑制大鼠癫痫样放电活动可能通过这一途径实现。
BACKGROUND: Ligustrazine can inhibit the activity of neurons in hippocampal neurons. It can effectively penetrate the blood-brain barrier after being absorbed in vivo, and is widely distributed in the cerebral cortex, brain stem, striatum, hippocampus, cerebellum, and midbrain. Objective: To investigate the effects of tetramethylpyrazine and its different drug concentrations on the structure of cerebral cortex neurons in rats with penicillin-induced seizures. Design: Randomized controlled trials. Unit: Department of Physiology, Xianning College of Medicine. MATERIALS: The experiment was performed at the Department of Anatomy, Tongji Medical College, Huazhong University of Science and Technology from September 2004 to March 2005. 40 healthy and clean SD rats were selected, either male or female, and the body weight was 200-250 g. They were randomly divided into 5 groups: surgical control group, penicillin-induced epilepsy group, and tetramethylpyrazine 10 mg/kg, 20 mg/kg, and 40 mg/kg group, 8 in each group. METHODS: Rats were anesthetized and exposed. The brain cortical recording area was exposed. BL-410 biological function experiment system was used to record left and right sides of brain electricity. Penicillin-induced penicillin-induced epilepsy group and ligustrazine were used. 10m g/kg, 20m g/kg, 40m The rats in g/kg group had epileptic discharges in cerebral cortex. In the surgical control group, the brain was taken 1h after anesthesia and craniotomy; in the penicillin-induced seizure group, brain was taken 1h after penicillin-induced epilepsy; Tetramethylpyrazine 10m g/kg, 20mg/kg, 40m g/kg group was stable after penicillin-induced seizure discharge. Then, intraperitoneal injection of tetramethylpyrazine (10 mg/kg, 20 mg/kg, 40 mg/kg) was given intraperitoneally, and the brain was taken when the inhibition was most obvious. Brain tissue sections were prepared, stained with hematoxylin and eosin, and observed under an optical microscope. MAIN OUTCOME MEASURES: Structural changes of cerebral cortical neurons in each group. RESULTS: All 40 rats were involved in the analysis of the results without any loss. 1 The morphological structure of cerebral cortical nerve cells in normal control rats was normal. 2 In the penicillin-induced epilepsy group, the structure of cerebral cortex neurons showed obvious changes, nuclear condensation, cytoplasmic dissolution, vacuolar structure, and no Nissl body in the cytoplasm. 3 Ligustrazine 10mg/kg group rats compared with the surgical control group, nuclear condensation, cytoplasm dissolved, vacuolar structure, reduced cytoplasmic Nissl body. 4 Compared with the control group, the rats in the TMP 20 mg/kg group had less vacuoles in the nerve cells, increased cytoplasm, and a small amount of Nissl body in the cytoplasm. Cell structure has improved. 5 Compared with the control group, the rats in the 40 mg/kg Ligustrazine group had large nucleus and circular staining, and there was a massive Nissl body in the cytoplasm, and the cell structure tended to be normal. Conclusion: The morphological structure of cerebral cortical neurons in rats with penicillin-induced seizures is abnormal. Tetramethylpyrazine at different doses can improve the morphological structure of cerebral cortical neurons to varying degrees, but the high-dose tetramethylpyrazine has a particularly significant improvement effect. Epileptiform discharge activity may be achieved through this approach.