A comparison of the toxicity of landfill leachate exposure at the seed soaking and germination stage

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To compare the toxicity of landfill leachate exposure at the early stages of seed soaking and germination on maize,a field experiment was conducted to evaluate the physiological aspects of growth,yield and potential clastogenicity of root-tip cells.The maizes were treated with leachate at levels of 2%,10%,20%,30% or 50% (WV).First,the change of physiological indexes,including chlorophyll (Chl),Malondialdehyde (MDA) and Reactive oxygen species (ROS) levels,combined with yield all showed that soaking with leachate,but not germination,generated a greater ecological risk on maize.After a soaking treatment of maize with 50% leachate,the Chl,MDA and ROS levels during a vigorous growth period were 47.3%,149.8% and 309.7%,respectively,of the control,whereas the yield decreased to 68.6% of the control.In addition,our results demonstrated that the leachate at lower levels could promote growth.This is mainly embodied in that the yield of maize treated with 10% leachate at the soaking stage increased to 116.0% of the control.Moreover,the cytological analysis experiment also demonstrated that the ecological risk of leachate still exists in both cases.Furthermore,the gray relational analysis showed that the ear row number and tassel branch number were the major factors affecting the yield of maize treated with 50% leachate at the stages of soaking and germination,respectively.In general,these results are helpful in understanding the phytotoxicity of leachate,which provides additional reference data for risk assessment and management of leachate.
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