〔目的〕研究一种适合现场使用的快速检测方法,用于对蚊虫携带登革病毒的初步筛查。〔方法〕采用双抗体夹心技术,建立上转换发光(UCP)免疫层析方法,筛查蚊体携带的登革病毒。〔结果〕该方法能在15min内对蚊虫携带的登革病毒进行快速筛查。〔结论〕建立的免疫层析方法简便、快速、敏感、特异,适合卫生检疫工作现场使用。 [Objective] To study a rapid detection method suitable for field use for the preliminary screening of mosquitoes carrying dengue virus. [Methods] The double antibody sandwich technique was used to establish up-conversion luminescence (UCP) immunochromatography to screen mosquito-borne dengue virus. [Result] The method can rapidly screen mosquito-borne dengue virus in 15 minutes. [Conclusion] The established immunochromatographic method is simple, rapid, sensitive and specific, and is suitable for the field of health and quarantine work.