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目的研究大肠癌的发生机制。方法采用聚合酶链式反应-单链构象多态性分析技术对大肠癌患者的肿瘤、正常组织及癌旁组织抑癌基因APC突变集中区MCR和抑癌基因MCC第10、12、15外显子的变化进行检测。结果大肠癌及癌分组织APC基因MCR15G、15H、15I三段的突变率显著高于正常组织，而肿瘤和痛分组织之间差异无显著性。MCC基因10、12、15三个外显子在大肠癌及癌旁组织的突变率与正常组之间差异有显著性，而肿瘤与癌旁之间差异无显著性。APC和MCC二者在癌组织中的总突变率高于APC和MCC单基因的突变率。结论APC和MCC突变检测有可能用于大肠癌早期基因诊断。
Objective To study the mechanism of colorectal cancer. METHODS: Polymerase chain reaction-single strand conformation polymorphism analysis was used to detect tumor suppressor gene APC mutation concentration area MCR and tumor suppressor gene MCC numbers 10, 12, and 15 in colorectal cancer patients. Changes in the child are detected. Results The mutation rate of APC gene MCR15G, 15H, 15I in colorectal cancer and cancerous tissue was significantly higher than that in normal tissue, but there was no significant difference between tumor and pain tissue. There were significant differences between the mutation rates of the three exons of MCC gene 10, 12, and 15 in colorectal cancer and adjacent tissues, and there was no significant difference between tumors and adjacent tissues. The total mutation rate of APC and MCC in cancer tissues was higher than that of APC and MCC single genes. Conclusion APC and MCC mutation detection may be used for early gene diagnosis of colorectal cancer.