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目的了解中俄边境地区蜱类自然感染斑点热群立克次体(Rickettsia japonica)的状况,为蜱传斑点热的监测和检测提供技术支持。方法采集鼠类寄生蜱,研磨后提取蜱DNA,利用PCR扩增斑点热群立克次体Omp A基因,对阳性产物进行序列分析。结果在满洲里地区,鼠类体表采集到的蜱类样本,均为全沟硬蜱(Ixodes persulcatus),从8只蜱中检测到斑点热群立克次核酸。序列分析结果表明,其中12号标本中检出的斑点热立克次体与Gen Bank数据库中已注册的斑点热群立克次核酸序列有高度的相似性(98%~99%)。结论应加强对内蒙古满洲里地区蜱传斑点热的监测和检测,防止蜱传疾病的发生。
Objective To understand the status of tick naturally infected Rickettsia japonica in China-Russia border area and provide technical support for the detection and detection of spot fever in ticks. Methods The murine parasitic ticks were collected, the tick DNA was extracted after grinding, and Omp A gene was amplified by PCR. The positive products were sequenced. Results In Manchuria, the ticks collected from the body surface of the rats were all Ixodes persulcatus. Spotted fever group Rickettsia was detected from 8 ticks. Sequence analysis showed that there was a high degree of similarity (98% -99%) between the spotted hot clones identified in GenBank and Gen Bank database. Conclusion The detection and detection of tick-borne fever in Manchuria, Inner Mongolia should be strengthened to prevent tick-borne diseases.