[Objective] This study aimed to investigate the preparation of red component in secondary metabolites of recombinant Hansenula anomala strain N6076 and its GC-MS detection. [Method] Thin-layer chromatography method was applied for large-scale preparation of red component in the secondary metabolites of recombinant H. anomala strain N6076. The red component was dissolved in anhydrous ethanol for GC-MS detection and chemical structure comparison in the database to identify its type. [Result] The red component is preliminarily identified as a quinone compound, while no compound with exactly the same structure as the red component has been found in WILEY, Nist and Nbs compound libraries. [Conclusion] This study laid the foundation for further NMR structure identification of the obtained red component and investigation of the relationship between its structure and biological effects. [Objective] This study aimed to investigate the preparation of red component in secondary metabolites of recombinant Hansenula anomala strain N6076 and its GC-MS detection. [Method] Thin-layer chromatography method was applied for large-scale preparation of red component in the secondary metabolites of recombinant H. anomala strain N6076. The red component was dissolved in anhydrous ethanol for GC-MS detection and chemical structure comparison in the database to identify its type. [Result] The red component was preliminarily identified as a quinone compound, while no compound with exactly the same structure as the red component has been found in WILEY, Nist and Nbs compound libraries. [Conclusion] This study laid the foundation for further NMR structure identification of the obtained red component and investigation of the relationship between its structure and biological effects.