论文部分内容阅读
目的:探讨金属蛋白酶解离素28(ADAM28)反义核酸(AS-ODN)治疗牙根发育不良疾病(CHTR)的可行性和效果。方法:应用反义核酸技术、基因重组、动物实验和酶联免疫吸附测定(ELISA)法检测ADAM28 AS-ODN、S-ODN和真核质粒对CHTR的不同效应。结果:用药后AS-ODN组患牙松动度明显减轻,牙周袋深度显著变浅,COL-Ⅱ、IL-1β、PGE2水平均显著下降,呈现炎症愈合趋势;S-ODN组和真核质粒组各项检测结果正相反,炎症呈显著上升趋势。结论:应用ADAM28 AS-ODN(200μmol/L)可有效治疗牙根发育不良疾病,但最佳药物浓度和用药时间还需进一步探讨。
Objective: To investigate the feasibility and effect of ADAM28 antisense oligonucleotide (AS-ODN) in the treatment of root dysplasia (CHTR). Methods: Different effects of ADAM28 AS-ODN, S-ODN and eukaryotic plasmid on CHTR were detected by antisense nucleic acid technique, gene recombination, animal experiments and ELISA. Results: After AS-ODN treatment, the degree of loosening of the tooth in AS-ODN group was significantly reduced, the depth of periodontal pocket was significantly lighter, the levels of COL-Ⅱ, IL-1β and PGE2 were significantly decreased, The test results of the group are the opposite, the inflammation showed a significant upward trend. Conclusion: ADAM28 AS-ODN (200μmol / L) can effectively treat the dysplasia of the root. However, the optimal drug concentration and time need to be further explored.