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本研究旨在建立骨髓涂片荧光免疫表型结合间期原位杂交技术(FICTION),为检测多发性骨髓瘤(MM)患者的分子细胞遗传学提供新方法。以骨髓涂片为载体,通过标记FITC的抗CD138抗体筛选浆细胞(PC),比较筛选的PC比例与形态学检测的PC比例之间的差异。同时采用8号染色体着丝粒探针行间期荧光原位杂交技术,检测PC的8号染色体异常情况。结果表明:骨髓涂片上荧光抗体筛选的PC比例与骨髓形态学检测的PC比例相比无统计学差异(p>0.05),9例患者中4例(44%)有8号染色体异常,其中8号染色体单体(-8)3例(33%),8号染色体三倍体(+8)1例(11%)。结论:骨髓涂片FICTION技术具有简便、特异、准确的特点,可用于MM分子遗传学异常的研究。
The aim of this study was to establish a fluorescence immunophenotypic interphase in situ hybridization (FICTION) technique in bone marrow smear, which provided a new method for the detection of molecular cytogenetics in patients with multiple myeloma (MM). The plasmacytoid (PC) was screened by FITC-labeled anti-CD138 antibody using a bone marrow smear as a carrier, and the difference between the screened PC ratio and the morphologically detected PC ratio was compared. At the same time, chromosome 8 centromere probe interphase fluorescence in situ hybridization was used to detect PC chromosome 8 abnormalities. The results showed that there was no significant difference (p> 0.05) in the proportion of PC screened by fluorescent antibody on bone marrow smears compared with that of bone marrow (p> 0.05), and 8 (44%) of 9 patients had chromosome 8 abnormality Three cases (33%) of chromosome 8 (-8) and one case of chromosome 8 (+8) (1%) on chromosome 8. Conclusion: The bone marrow smear FICTION technique is simple, specific and accurate. It can be used in the study of MM molecular abnormality.