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目的研究黄芩苷(BAI)对2型糖尿病(T2DM)大血管病变的保护作用,并探讨其可能机制。方法将30只健康成年雄性Wistar大鼠随机分为3组:对照组、模型组、中药组,每组10只。对照组大鼠喂以标准饲料且不做任何处理;其余各组大鼠通过高糖高脂饲料喂养+腹腔注射链脲佐菌素(STZ)诱发糖尿病,同时中药组大鼠每日给予BAI 10mg/kg灌胃;而对照组和模型组给予相当量的0.9%氯化钠注射液灌胃。8周后检测各组大鼠血糖(BS)、血脂(TC,TG,HDL,LDL)水平,细胞间黏附分子-1(ICAM-1),血管细胞粘附分子-1(VCAM-1),核转录因子-kappa B(NF-κB)及单核细胞趋化蛋白-1(MCP-1)的表达水平。结果模型组大鼠BS,TC,TG,LDL,ICAM-1,VCAM-1,NF-κB及MCP-1的表达水平较空白组均显著升高,LDL的表达水平较空白组显著降低,中药组相关指标较模型组均降低,差异有统计学意义(P<0.05)。结论 BAI对T2DM大鼠受损血管具有保护作用,其机制可能与降低血糖、血脂,改善铁代谢紊乱有关。
Objective To study the protective effect of baicalin (BAI) on type 2 diabetes mellitus (T2DM) macrovascular disease and its possible mechanism. Methods Thirty healthy adult male Wistar rats were randomly divided into three groups: control group, model group and traditional Chinese medicine group, with 10 rats in each group. The rats in the control group were fed with standard diet without any treatment. The rats in the other groups were fed with high glucose and high fat diet and streptozotocin (STZ) intraperitoneally to induce diabetes mellitus. At the same time, / kg gavage; while the control group and model group given a considerable amount of 0.9% sodium chloride injection gavage. After 8 weeks, the levels of blood glucose (BS), blood lipids (TC, TG, HDL, LDL), ICAM-1, VCAM- The expression levels of nuclear transcription factor-kappa B (NF-κB) and monocyte chemoattractant protein-1 (MCP-1) Results The expression of BS, TC, TG, LDL, ICAM-1, VCAM-1, NF-κB and MCP-1 in model group were significantly higher than those in blank group, while the expression of LDL in model group was significantly lower than that in blank group Group related indicators lower than the model group, the difference was statistically significant (P <0.05). Conclusions BAI has a protective effect on the damaged blood vessels in T2DM rats. The mechanism may be related to reducing blood glucose, blood lipid and improving iron metabolism disorder.