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目的:探讨死亡相关蛋白激酶(death associated protein kinase,DAPK)甲基化及人乳头瘤病毒(human papilloma virus,HPV)感染与新疆维吾尔族妇女宫颈病变的相关性,为维吾尔族妇女宫颈病变的诊断和治疗提供参考依据。方法:选择新疆维吾尔自治区人民医院2008年至2009年收治的宫颈非癌组织30例、宫颈上皮内瘤变(CIN)Ⅰ30例、CINⅡ/Ⅲ30例、宫颈鳞癌组织30例患者的组织标本为研究对象,运用MSP法检测宫颈组织中DAPK基因启动子甲基化水平及PCR检测各组织中HPV的感染情况,对二者进行相关性分析。结果:(1)宫颈非癌组织、CINⅠ、CINⅡ/Ⅲ、宫颈鳞癌组织中DAPK基因甲基化的检出率分别为3.33%、10%、36.7%、63.3%,宫颈鳞癌组的甲基化率明显高于其它三组,差异有统计学意义(P<0.05);(2)四组HPV的阳性率分别为43.3%、56.7%、76.7%、93.3%,宫颈鳞癌组的HPV感染率明显高于其它三组,差异有统计学意义(P<0.05),HPV的感染率和宫颈病变程度成正相关(P<0.05)。(3)宫颈病变组织中DAPK基因的甲基化水平与HPV-DNA阳性率呈正相关。结论:DAPK基因启动子的甲基化及HPV感染在新疆维吾尔族妇女宫颈病变尤其是宫颈鳞癌的发生过程中可能起重要作用,二者呈正相关,联合检测二者可能有助于新疆维吾尔族妇女宫颈鳞癌的诊断。
Objective: To investigate the correlation between death associated protein kinase (DAPK) methylation and human papilloma virus (HPV) infection and cervical lesions in Uygur women in Xinjiang and to provide a diagnosis of cervical lesions in Uighur women And provide a reference for treatment. Methods: Tissue samples of 30 cases of cervical non-cancerous tissue, 30 cases of cervical intraepithelial neoplasia (CIN), 30 cases of CINⅡ / Ⅲ and 30 cases of cervical squamous cell carcinoma were selected from People’s Hospital of Xinjiang Uygur Autonomous Region between 2008 and 2009 The MSP method was used to detect the promoter methylation level of DAPK gene in cervical tissue and PCR to detect the HPV infection in each tissue. Results: (1) The detection rates of DAPK methylation in cervical non-cancerous tissues, CINⅠ, CINⅡ / Ⅲ and cervical squamous cell carcinoma were 3.33%, 10%, 36.7% and 63.3% (2) The positive rates of HPV in the four groups were 43.3%, 56.7%, 76.7%, 93.3%, respectively.The positive rate of HPV in the cervical squamous cell carcinoma group was significantly higher than that in the other three groups (P <0.05) The infection rate was significantly higher than the other three groups, the difference was statistically significant (P <0.05). The infection rate of HPV was positively correlated with the degree of cervical lesions (P <0.05). (3) The methylation level of DAPK gene in cervical lesions was positively correlated with the positive rate of HPV-DNA. CONCLUSION: Methylation of DAPK promoter and HPV infection may play an important role in the pathogenesis of cervical lesions in Uyghur women, especially cervical squamous cell carcinoma in Xinjiang. The combination of them may be of help to the Uyghur Diagnosis of cervical squamous cell carcinoma in women.