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以野生型p53cDNA片段作探针，采用DNA分子印迹杂交（Southernblot）技术检测20例原发性肺癌和10例肺良性病变p53基因缺失与重排。肺癌组阳性率为30％；肺良性病变组均为阴性。同时采用聚合酶链反应结合限制性片段长度多态性分析（PCR-RFLP）检测20例原发性肺癌，2例肺转移癌以及它们相应的非癌肺组织p53基因248位密码子点突变。结果发现，4例原发性肺癌和2例肺转移癌有p53基因248位密码子点突变，非癌肺组织均为阴性。P53基因缺失和突变与肿瘤大小、分期、非小细胞肺癌病理分型无关（P＞0．05），与肿瘤转移有关（P＜0．05）。提示：p53基因缺失和突变与肺癌的发生和预后密切相关。
The wild-type p53 cDNA fragment was used as a probe, and the DNA deletion and rearrangement of p53 gene in 20 cases of primary lung cancer and 10 cases of benign lung lesions were detected by DNA molecular blot hybridization (Southern blot) technique. The positive rate in lung cancer group was 30%; benign lung disease group was negative. At the same time, polymerase chain reaction and restriction fragment length polymorphism analysis (PCR-RFLP) were used to detect 248 codon mutations of p53 gene in 20 primary lung cancers, 2 lung metastatic carcinomas, and their corresponding non-cancer lung tissues. The results showed that there were 248 codon mutations in the p53 gene in 4 cases of primary lung cancer and 2 cases of metastatic lung cancer, and were negative in non-cancerous lung tissue. P53 gene deletions and mutations were not associated with tumor size, stage, pathological type of non-small cell lung cancer (P>0.05), and were related to tumor metastasis (P<0.05). Tip: The deletion and mutation of p53 gene are closely related to the occurrence and prognosis of lung cancer.