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目的建立黄连不同部位中盐酸药根碱、盐酸非洲防己碱、盐酸表小檗碱、盐酸黄连碱、盐酸巴马汀和盐酸小檗碱的测定方法。方法色谱条件为Phenomenex Gemini-NX C18色谱柱(250 mm×4.6 mm,5μm);流动相为乙腈-30 mmol/L碳酸氢铵溶液(每1 000 mL碳酸氢铵溶液含7 mL氨水及1 mL三乙胺);体积流量1.0 mL/min;检测波长270 nm;柱温30℃。结果黄连须根、根茎、叶中6个生物碱量具有较明显差异。经比较,黄连适宜在移栽后第4年采挖,7～10月的最佳采挖月份是10月。结论该法简便、准确、分离效果好,适用于黄连主要生物碱的定量分析。
OBJECTIVE To establish a method for the determination of jatrorrhizine hydrochloride, tetrandrine hydrochloride, berberine hydrochloride, berberine hydrochloride, palmatine hydrochloride and berberine hydrochloride in different parts of Coptis chinensis. Methods The chromatographic conditions were Phenomenex Gemini-NX C18 column (250 mm × 4.6 mm, 5 μm). The mobile phase consisted of acetonitrile-30 mmol / L ammonium bicarbonate solution (containing 7 mL ammonia water per 1 000 mL ammonium bicarbonate solution and 1 mL Triethylamine); volume flow 1.0 mL / min; detection wavelength 270 nm; column temperature 30 ℃. Results Coptis root, rhizome, leaf alkaloids in the amount of more significant differences. After comparison, Coptis suitable for excavation in the first 4 years after transplanting, 7 to October the best mining month is October. Conclusion The method is simple, accurate, good separation effect, suitable for the quantitative analysis of the main alkaloids in Coptis.