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为了研究生药挥发性成分在血中的浓度,以茴香中挥发油的主要成分茴香脑为例,探讨了给药小鼠血浆含量的简便测定法,并且探讨了生药对其浓度是否有影响。 以HPLC测定血浆中茴香脑的含量,首先在小鼠的分离血浆中添加茴香脑,以氯仿提取,观察回收率。其次对小鼠经口给予茴香脑,一定时间后采血测定血浆中的浓度。进一步用50％乙醇提取茴香。经口给
For the concentration of the volatile components in the blood of postgraduate students, taking anethole, the main component of volatile oil in fennel, as an example, a convenient method for measuring the plasma concentration in mice was investigated, and whether or not the crude drug had an effect on its concentration was discussed. The content of anethole in plasma was determined by HPLC. Anethole was firstly added to the isolated plasma of the mouse and extracted with chloroform to observe the recovery rate. Next, the mice were given anethole orally, and blood was collected after a certain period of time to determine the plasma concentration. Further fennel was extracted with 50% ethanol. Oral delivery