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抗红细胞非凝集型抗体是建立“全血免疫分析系统”的必要条件。为此，我们采用小鼠骨髓瘤细胞ＳＰ２／０和纯化人红细胞膜血型糖蛋白Ａ（ＧｌｙｃｏｐｈｏｒｉｎＡ，ＧＰＡ）免疫的Ｂａｌｂ／ｃ小鼠的脾细胞融合，获得了一株分泌抗ＧＰＡ非凝集型单克隆抗体的杂交瘤细胞系——３Ｃ５。３Ｃ５分泌的ＭｃＡｂ为ＩｇＧ１亚类，κａｐｐａ型轻链，能够识别和结合红细胞膜的血型糖蛋白Ａ和血型糖蛋白Ｂ（ＧｌｙｃｏｐｈｏｒｉｎＢ，ＧＰＢ），说明其识别的是ＧＰＡ和ＧＰＢ分子所共有的抗原决定簇。该ＭｃＡｂ可以特异性地与红系细胞的膜抗原结合，其结合不受血型系统的限制，也不引起红细胞的直接凝集，可应用于“全血免疫分析系统”。此外，对红细胞的分化研究和红白血病的特异性诊断也有重要的应用价值。
Anti-erythrocyte non-agglutinating antibodies is necessary to establish a “whole blood immunoassay system.” To this end, we fused splenocytes of Balb / c mice immunized with mouse myeloma cells SP2 / 0 and purified human erythrocyte glycoprotein A (GPA) to obtain a secreting anti-GPA non-agglutinating single The McAb secreted by the clone antibody-3C5.3C5 is an IgG1 subclass and a κappa-type light chain, which can recognize and bind the glycophorin A and glycoprotein B (GPB) of the erythrocyte membrane, indicating that the recognition Is an antigenic determinant common to GPA and GPB molecules. The McAb can specifically bind to the membrane antigen of erythroid cells, the binding of which is not restricted by the blood group system, nor the direct agglutination of erythrocytes, and can be applied to a “whole blood immunoassay system.” In addition, the differentiation of erythrocytes and erythroleukemia-specific diagnosis also has important application value.