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目的研究P21在宫颈癌和相应癌旁组织中的表达以及与临床病理参数之间的关系。方法收集2010年1月—2016年2月巴彦淖尔市医院就诊的子宫颈癌患者40例,从宫颈癌组织及相应癌旁组织中提取RNA后反转录成c DNA,以GAPDH作为内参,利用PT-PCR技术对P21基因的表达情况进行分析计量资料采用t检验,P<0.05为差异有统计学意义。结果宫颈癌组织和相应癌旁组织中P21 mRNA对GAPDH的相对丰度值分别是(0.16±0.26)和(0.30±0.28),两组表达差异有统计学意义(P<0.05),并且宫颈癌组织中P21 mRNA表达水平与病理分化程度、国际妇产科联盟(international federation of gynecology and obstetrics,FIGO)分期、淋巴结转移、浸润深度有关,与组织学类型和年龄无关。结论 P21在宫颈癌组织中低表达,可能与宫颈癌的发生发展密切相关。
Objective To investigate the expression of P21 in cervical cancer and its corresponding paracancerous tissues and its relationship with clinicopathological parameters. Methods From January 2010 to February 2016, 40 patients with cervical cancer from Bayannur Hospital were enrolled and RNA was reverse transcribed into c DNA from cervical cancer tissues and corresponding paracancerous tissues. GAPDH was used as an internal control, The expression of P21 gene was analyzed by PT-PCR. The measurement data were analyzed by t-test. P <0.05 was considered statistically significant. Results The relative abundance of P21 mRNA in GAC and cervical cancer tissue was (0.16 ± 0.26) and (0.30 ± 0.28), respectively, with significant difference between the two groups (P <0.05) The expression level of P21 mRNA in the tissue correlated with the degree of pathological differentiation, the level of FIGO staging, lymph node metastasis and depth of invasion, but not with histological type and age. Conclusion The low expression of P21 in cervical cancer may be closely related to the occurrence and development of cervical cancer.