论文部分内容阅读
用不同乳腺癌细胞系475A、231和乳癌患者的原发瘤组织制备的单细胞悬液免疫BALB/C小鼠,取其脾细胞与小鼠骨髓瘤细胞SP 2/0融合,融合率为50％。获得3株与乳腺癌细胞系有反应的杂交瘤,择其1株培养上清与免疫原呈强阳性反应而与混合淋巴
BALB/C mice were immunized with single cell suspensions prepared from primary tumor tissues of different breast cancer cell lines 475A, 231 and breast cancer patients. The spleen cells were fused with mouse myeloma cells SP 2/0 and the fusion rate was 50. %. Three hybridomas that responded to breast cancer cell lines were obtained, and one culture supernatant was selected to be strongly positive with the immunogen and mixed with the lymph