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至引言单肢递质及其代谢物含量的变化与人和动物机体的各种应激状态及许多疾病有关。对其准确、灵敏而特异地检测一直是药物学、临床医学等领域中重要的研究。高效液相色谱紫外检测，灵敏度只能达毫微克水平；高效液相色谱荧光检测，待测组分通常需要柱前（或柱后）衍生而且不
INTRODUCTION Changes in monoamine neurotransmitters and their metabolites have been linked to various stress states in humans and animals and to many diseases. Its accurate, sensitive and specific detection has been an important study in the fields of pharmacy, clinical medicine and so on. High-performance liquid chromatography with UV detection, the sensitivity can only reach nanograms level; high performance liquid chromatography fluorescence detection, test components usually need to pre-column (or post-column) derived and not