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硫酸软骨素是从动物组织中提取获得的粘多糖。地方标准采用重量法测定其含量,但操作繁复费时。作者试用交流示波极谱滴定法,将样品加碱加热解离出 SO_4~(2-),经过滤,滤液中定量加入过量的 BaCl_2标准液,利用形成稳定的 Ba~(2+)-PEG-NaTPB 三元络合物的反应,在 HAC-NaAc-PEG 底液中以 NaTPB 标准液滴定剩余的 Ba~(2+)。根据 dE/dt—E 曲线上 NaTPB 切口的出现指示滴定终点。测定五份同一样品,相对标准偏差为±0.6%,回收率为98.3～100.0%。测定结果和重量法一致,可替代目前采用的地方标准方法。
Chondroitin sulfate is a mucopolysaccharide extracted from animal tissues. Local standards using gravimetric determination of its content, but the operation complicated and time-consuming. The authors tried to exchange the oscillopolarographic titration method, the sample was heated with alkali to dissociate SO_4 ~ (2-), filtered, the filtrate was quantitatively added excess BaCl_2 standard solution, the use of the formation of stable Ba ~ (2 +) - PEG -NaTPB ternary complex, the remaining Ba 2+ was titrated with NaTPB standard solution in HAC-NaAc-PEG. The end of the titration was indicated by the appearance of a NaTPB nick on the dE / dt-E curve. Five identical samples were determined with a relative standard deviation of ± 0.6% and a recovery of 98.3 ~ 100.0%. The measurement results are consistent with the gravimetric method, which can replace the local standard methods currently used.