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目的:观察英连金化浊解毒方对慢性萎缩性胃炎伴中度IM或轻度Dys大鼠的CD44V6基因蛋白表达的影响。方法:将120只大鼠随机分为空白组、模型组、大、中、小剂量组及干预组。采用N-甲基-N-硝基N-亚硝基胍配合饥饱失常诱导造模。造模后分组治疗,24周后处死全部实验大鼠并通过TUNEL细胞凋亡检测法对CD44V6指标进行检测。结果:与空白组相比,模型组CD44V6表达率明显增高,差异有统计学意义(P<0.05),英连金化浊解毒方大、中、小剂量组CD44V6表达率同模型组两两比较均降低,差异均具有统计学意义(P<0.05)。结论:英连金化浊解毒方(大、中、小剂量组)能够明显改善胃黏膜组织病理状况,降低Cd44v6基因蛋白表达。
Objective: To observe the effect of “Yinglian Jinhuazhuojiedu Decoction” on CD44V6 gene protein expression in chronic atrophic gastritis with moderate IM or mild Dys. Methods: 120 rats were randomly divided into blank group, model group, large, medium and small dose groups and intervention group. The use of N-methyl-N-nitro-N-nitrosoguanidine with starvation induced abnormal modeling. All the rats were killed after 24 weeks and the CD44V6 index was detected by TUNEL apoptosis assay. Results: Compared with the blank group, the expression rate of CD44V6 in the model group was significantly increased (P <0.05), and the expression rate of CD44V6 in Yinglianjinhuazhuojiedu Fang, medium and small dose groups was significantly higher than that in the model group All the differences were statistically significant (P <0.05). CONCLUSION: Yinglianjinhuazhuo Jiedu Decoction (large, medium and low dose groups) can significantly improve the pathological conditions of gastric mucosa and reduce the expression of Cd44v6 gene.