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目的通过系统的实验室检测发现并确认1例输入性基孔肯雅病例。方法根据基孔肯雅病毒基因序列设计引物和荧光标记探针,利用实时荧光RT-PCR和常规RT-PCR检测方法对广东出入境检验检疫局发现的1例入境发热患者的血清样本进行检测,并对RT-PCR扩增产物进行核苷酸序列测定。结果通过实时荧光RT-PCR和常规RT-PCR 2种实验方法在该病例标本中检出基孔肯雅病毒核酸;对RT-PCR扩增产物进行序列测定,测出的521个碱基与GeneBank数据库公布的基孔肯雅病毒核酸序列进行比对,结果显示同源性高达99%。结论结合该患者临床症状、实验室检测结果及流行病学调查结果,判断该病例为输入性基孔肯雅病例。
Objective To identify and confirm one case of enterovirus Chikungunya through systematic laboratory tests. Methods According to the sequence of Chikungunya virus, primers and fluorescently labeled probes were designed. Serum samples of one case of imported fever were detected by real-time fluorescent RT-PCR and routine RT-PCR. The nucleotide sequence of the RT-PCR amplification product was determined. Results Chikungunya virus nucleic acid was detected in the specimens by real-time fluorescence RT-PCR and conventional RT-PCR. The sequence of RT-PCR amplification products was determined. The 521 base pairs were identical to those of GeneBank Database published Chikungunya virus nucleic acid sequence alignment, the results showed that homology up to 99%. Conclusion Combined with the clinical symptoms, laboratory test results and epidemiological findings, this case is judged to be imported chikungunya cases.