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植物名称:小金海棠(molus xiaojinensis)材料类别:小金海棠的种子,用冷水浸泡吸胀,经0.1％升汞消毒15分钟后,无菌水冲洗干净,接种子琼脂培养基上,2～3周后发芽(发芽率只有20～30％左右),然后切取子叶进行离体培养。培养条件:每升MS基本培养基,附加6-BAI～2mg/L(单位下同),NAA0.1～0.2,LH100,肌醇100,蔗糖3％,琼脂粉0.5％,培养温度26±2℃,每天光照8～10小时,光照强度2000 lx。生长与分化情况:子叶接种子培养基上4～5周后,子叶长大变厚呈绿色,近轴面诱导出绿色芽点,逐渐分化成丛生芽,每片子叶可得3～10个绿色
Botanical Name: molus xiaojinensis Material Category: Begonia seedlings, swollen with cold water soaked with 0.1% mercuric chloride for 15 minutes, sterile water rinse, seeded on agar medium, 2 to 3 weeks After germination (germination rate of only about 20 to 30%), and then cut cotyledons for in vitro culture. Culture conditions: MS medium supplemented with 6-BAI ~ 2mg / L NAA0.1 ~ 0.2, LH100, inositol 100, sucrose 3%, agar powder 0.5%, culture temperature 26 ± 2 ℃, daily light 8 to 10 hours, light intensity 2000 lx. Growth and differentiation: Cotyledon inoculated medium 4 to 5 weeks later, the cotyledons grow thick and green, axon induced green buds, and gradually differentiated into clusters of buds, each leaf can have 3 to 10 green