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目的研究丹参多酚酸对心肌缺血损伤大鼠心肌酶与炎症因子水平的影响。方法按照体重将SD雄性大鼠随机分为4组:假手术组、模型组和低高2个剂量(丹参多酚酸18,36 mg·kg-1)实验组,每组15只,腹腔注射给药。假手术组和模型组按同等剂量注射蒸馏水,给药第4天,结扎冠状动脉左前降支建立心肌缺血模型。术后6 h,眼内眦取血,以酶联免疫吸附法检测血清肌酸激酶(CK)、肌酸激酶同工酶(CK-MB)和乳酸脱氢酶(LDH)水平;于24 h取心脏,苏木素-伊红(HE)染色,光学显微镜下观察心肌组织病理变化,酶联免疫吸附法检测血清血肌肌钙蛋白(c Tn T)、肿瘤坏死因子α(TNF-α)和心肌组织匀浆髓过氧化物酶(MPO)含量。结果心肌缺血6 h后,假手术组CK为(529.61±141.93)U·L~(-1),CK-MB为(708.12±385.93)U·L~(-1),LDH为(330.12±158.38)U·L~(-1);模型组CK为(996.42±413.42)U·L~(-1),CK-MB为(1346.11±558.30)U·L~(-1),LDH为(520.12±154.76)U·L~(-1);低高2个剂量实验组CK分别为(499.45±159.33),(514.91±98.82)U·L~(-1),CK-MB分别为(831.42±385.11),(592.10±206.32)U·L~(-1),LDH分别为(462.62±229.68),(437.72±175.80)U·L~(-1);与假手术组相比,模型组大鼠血清CK、CK-MB、LDH水平明显升高,差异有统计学意义(P<0.05);与模型组比较,低高2个剂量实验组能明显降低大鼠血清CK、CK-MB的含量,差异有统计学意义(均P<0.05)。心肌缺血24 h,假手术组和模型组c Tn T分别为(294.12±55.10),(513.00±69.71)pg·m L~(-1),差异有统计学意义(P<0.05);给药后,低高2个剂量实验组c Tn T分别为(431.12±106.00),(338.80±76.92)pg·m L~(-1),与模型组比较,高剂量实验组差异有统计学意义(P<0.05)。术后24 h,假手术组和模型组TNF-α分别为(118.90±17.58),(156.00±28.24)pg·m L~(-1),差异有统计学意义(P<0.05);给药后,低高2个剂量实验组TNF-α分别为(136.05±34.00),(125.06±30.13)pg·m L~(-1),与模型组比较,高剂量实验组差异有统计学意义(P<0.05)。假手术组和模型组MPO分别为(32.25±3.75),(104.83±22.87)U·L~(-1),差异有统计学意义(P<0.01);给药后,低高2个剂量实验组MPO分别为(91.70±21.57),(68.00±18.47)U·L~(-1),与模型组比较,高剂量实验组差异有统计学意义(P<0.01)。结论丹参多酚酸具有心肌保护作用,能明显减轻心肌细胞损伤,减少心肌组织炎性细胞浸润,这可能与降低TNF-α、MPO等炎性介质水平有关。
Objective To investigate the effect of Salviae miltiorrhizae polyphenols on myocardial enzymes and inflammatory cytokines in rats with myocardial ischemic injury. Methods SD male rats were randomly divided into 4 groups according to body weight: sham-operation group, model group and low dose group (Salviae micropytic acid 18,36 mg · kg-1) Administration. The rats in sham-operation group and model group were injected distilled water at the same dose. On the fourth day after administration, left anterior descending coronary artery was ligated to establish myocardial ischemia model. At 6 h after operation, blood was collected from the eyes and serum creatine kinase (CK), creatine kinase (CK-MB) and lactate dehydrogenase (LDH) levels were measured by enzyme-linked immunosorbent assay The hearts were stained with hematoxylin-eosin (HE), the pathological changes of myocardium were observed under a light microscope, and the levels of serum cTn, TNF-α and myocardium were detected by enzyme-linked immunosorbent assay (ELISA) Tissue homogenate myeloperoxidase (MPO) content. Results After 6 h of myocardial ischemia, CK was (529.61 ± 141.93) U · L -1, CK-MB was (708.12 ± 385.93) U · L -1, LDH was (330.12 ± (CK), CK (1346.11 ± 558.30) U · L -1, LDH was ( 520.12 ± 154.76) U · L ~ (-1). CK in the two groups with low dose and high dose were respectively (499.45 ± 159.33), (514.91 ± 98.82) U · L ~ (-1) and CK- ± 385.11, 592.10 ± 206.32 U · L -1, and LDH were (462.62 ± 229.68) and (437.72 ± 175.80) U · L -1, respectively. Compared with sham operation group, model group The levels of CK, CK-MB and LDH in serum of rats were significantly increased (P <0.05). Compared with the model group, the experimental group of low dose and high dose of 2 doses significantly reduced the levels of serum CK and CK-MB Content, the difference was statistically significant (all P <0.05). Twenty-four hours after myocardial ischemia, cTn T was (294.12 ± 55.10) and (513.00 ± 69.71) pg · m L -1 in sham operation group and model group, respectively, with significant difference (P <0.05) After treatment, the cTn T values in the two experimental groups with low and high doses were (431.12 ± 106.00) and (338.80 ± 76.92) pg · m L -1, respectively. Compared with the model group, the differences were statistically significant (P <0.05). At 24 h after operation, the TNF-α levels in the sham operation group and model group were (118.90 ± 17.58) and (156.00 ± 28.24) pg · m L -1, respectively, with statistical significance (P <0.05) After treatment, the TNF-α levels in the two low-dose and high-dose groups were (136.05 ± 34.00) and (125.06 ± 30.13) pg · m L -1, respectively. Compared with the model group, the difference was statistically significant P <0.05). The MPO of the sham group and the model group were (32.25 ± 3.75) and (104.83 ± 22.87) U · L -1, respectively, with statistical significance (P <0.01) (91.70 ± 21.57) and (68.00 ± 18.47) U · L ~ (-1), respectively. Compared with the model group, the experimental group had significant difference (P <0.01). Conclusion Salviae miltiorrhizae polyphenols have protective effect on myocardium, which can significantly reduce the injury of myocardial cells and decrease the infiltration of inflammatory cells in myocardium, which may be related to the decrease of inflammatory mediators such as TNF-α and MPO.