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The pre-calling behavior of female adults of the common cutworm, Spodoptera litura (Lepidoptera: Noctuidae) collected in southe Japan and southeaste Asian countries was investigated. Most females started calling on days 1-4 after adult emergence. In three populations obtained in Saga prefecture in Kyushu mainland, Japan, two different pattes of pre-calling period (PCP) were observed: one was the above-mentioned patte shown by most individuals, and the other was a patte in which females sexually matured within several hours after emergence. Diel periodicity was shown in the time of calling activity, and its patte varied among the populations. Pre-calling period was stable over successive generations in the laboratory.