论文部分内容阅读
采用比较简便的提纯方法,从300g人脑中得到凝胶电泳纯的肌酸激酶BB同工酶(Creatine kinase BB isoenzyme,CK-BB)24mg,比活力高达1104u/mg蛋白,酶活力回收66％。同时,制备兔抗人CK-BB抗血清。经~(125)I联结标记法标记CK-BB,比放射性8288kBq/μg,50％结合率的CK-BB抗血清稀释度1:25000,亲和常数(K值)3.0×10~9mol/L,并具有理想的抗体特异性,可适用于CK-BB的放射免疫分析(RIA)。此外,还测定了CK-BB的部分物化性质和免疫学性质。
Using a relatively simple purification method, 24 mg of pure creatine kinase BB isoenzyme (CK-BB) was obtained from 300 g human brain, and the specific activity was as high as 1104 u / mg protein, and the enzyme activity was recovered by 66% . At the same time, rabbit anti-human CK-BB antiserum was prepared. CK-BB was labeled by ~ (125) I labeling method, the dilution of CK-BB was 1:25000 and the affinity constant (K value) was 3.0 × 10-9mol / L compared with that of radioactive 8288kBq / , And has ideal antibody specificity, suitable for radioimmunoassay (RIA) of CK-BB. In addition, some physicochemical and immunological properties of CK-BB were also determined.