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目的探讨替米沙坦和血管紧张素Ⅱ对乳鼠胸主动脉平滑肌血管紧张素转化酶2表达的影响。方法应用蛋白免疫印迹法检测血管紧张素转化酶2蛋白的表达,以逆转录聚合酶链反应法检测血管紧张素转化酶2mRNA表达。结果在10-8～10-5mol/L浓度范围内,替米沙坦呈浓度依赖性上调血管紧张素转化酶2蛋白的表达,在0～24h时间内,10-6mol/L替米沙坦呈时间依赖性促进血管紧张素转化酶2的蛋白表达,并促进血管紧张素转化酶2mRNA的表达;在10-8～10-5mol/L浓度范围内,血管紧张素Ⅱ呈浓度依赖性下调血管紧张素转化酶2蛋白的表达;10-6mol/L替米沙坦能明显地拮抗10-6mol/L血管紧张素Ⅱ对血管紧张素转化酶2蛋白和mRNA表达的抑制作用。结论替米沙坦能促进胸主动脉平滑肌血管紧张素转化酶2蛋白和mRNA的表达,并能明显拮抗血管紧张素Ⅱ对血管紧张素转化酶2蛋白和mRNA表达的抑制作用。
Objective To investigate the effect of telmisartan and angiotensin Ⅱ on the expression of angiotensin-converting enzyme 2 in thoracic aorta smooth muscle in neonatal rats. Methods The expression of angiotensin-converting enzyme 2 protein was detected by Western blotting. The expression of angiotensin-converting enzyme 2 mRNA was detected by reverse transcription-polymerase chain reaction. Results In the concentration range of 10-8 ~ 10-5mol / L, telmisartan up-regulated angiotensin-converting enzyme 2 protein expression in a concentration-dependent manner. During the period of 0 ~ 24h, 10-6mol / L telmisartan In a time-dependent manner, the protein expression of angiotensin-converting enzyme 2 was promoted and the expression of angiotensin-converting enzyme 2 mRNA was promoted. In the concentration range of 10-8 ~ 10-5mol / L, angiotensinⅡdecreased in a concentration-dependent manner The expression of Angiotensin-Converting Enzyme 2 protein and 10-6mol / L Telmisartan significantly inhibited the inhibitory effect of 10-6mol / L Angiotensin II on angiotensin-converting enzyme 2 protein and mRNA expression. Conclusion Telmisartan can promote the expression of angiotensin-converting enzyme 2 protein and mRNA in the thoracic aorta smooth muscle and inhibit the inhibitory effect of angiotensin Ⅱ on angiotensin-converting enzyme 2 protein and mRNA expression.