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目的 针对海上血液储运后急需解决的质量评价标准缺失的难题,建立定量的血细胞能量代谢平台和技术,确定血细胞效能指标和定量评价标准,将“功能剂量”引入血细胞的质量判定,为完善海上血液储运规范提供有益补充.方法 采用细胞能量代谢实时检测技术细胞外流量(XF)分析技术检测红细胞糖酵解代谢过程中细胞外酸化率(ECAR)值的变化,反映海上航行对储运红细胞糖酵解水平的影响,与正常条件下保存的样品进行自身对照.并结合ATP含量检测试剂盒及游离血红蛋白检测试剂盒进行互相验证.结果 确定了XF技术能实时、定量检测红细胞的糖酵解水平.通过检测两次出海后血液样品的血液质量评价指标和能量学指标的变化,判断环渤海湾航行的血液样品各指标无明显变化,样品保存良好;环太平洋航行的样品能量学指标明显下降,建议弃用.结论将XF技术应用到红细胞糖酵解的测定中,在活细胞水平对血液进行高通量多指标动态检测,为海上储运后的血液质量进行综合系统评价提供了理论依据.“,”Objective To evaluate the quality of blood after marine storage and transportation,to establish a quantitative energy metabolism platform supported by extracellular flux(XF)technique to determine the glycolysis level of red blood cells(RBC), and to introduce the “functional dose”, as a valuable supplement, into the quality evaluation of RBCs after marine storage and transportation.Methods The extracellular acidification rate(ECAR)of erythrocyte glycolysis was detected by XF.The levels of glycolysis, ATP contents and free hemoglobin of RBCs were detected before and after marine navigation.RBC suspensions stored at 4℃in a blood bank on land served as control.Results The results showed that the glycolysis level of RBCs was detected by XF.Indicators of blood quality and energy metabolism of RBCs were detected in the twice marine storage and transportation.Blood samples transported around the Gulf of Bohai had no significant change,indicating that the samples were well preserved.The energy indicators of blood samples navigated in the Pacific Ocean decreased significantly,and it was recommended that the samples be discarded.Conclusion XF technique has been applied to the measurement of erythrocyte glycolysis,enabling high-throughput and multi-indicator dynamic detection of viable cells.The energy metabolism of erythrocyte should be recommended as an important indicator for evaluating blood after marine storage and transportation.