Turnover of diacylglycerol kinase 4 by cytoplasmic acidification induces vacuole morphological chang

来源 :植物学报(英文版) | 被引量 : 0次 | 上传用户:lzl1988
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
Pear has an S-RNase-based gametophytic self-incompatibility (SI) system. Nuclear DNA degrada-tion is a typical feature of incompatible pollen tube death, and is among the many physiological func-tions of vacuoles. However, the specific changes that occur in vacuoles, as well as the associated regulatory mechanism in pear SI, are currently un-clear. Although research in tobacco has shown that decreased activity of diacylglycerol kinase (DGK) results in the morphological change of pollen tube vacuole, whether DGK regulates the pollen tube vacuole of tree plants and whether it occurs in SI response, is currently unclear. We found that DGK activity is essential for pear pollen tube growth, and DGK4 regulates pollen tube vacuole morphology following its high expression and deposition at the tip and shank edge of the pollen tube of pear. Specifically, incompatible S-RNase may induce cytoplasmic acidification of the pollen tube by inhibiting V-ATPase V0 domain a1 subunit gene ex-pression as early as 30 min after treatment, when the pollen tube is still alive. Cytoplasmic acidification induced by incompatible S-RNase results in reduced DGK4 abundance and deposition, leading to mor-phological change of the vacuole and fragmentation of nuclear DNA, which indicates that DGK4 is a key factor in pear SI response.
其他文献
Variant selection during phase transformation creates a specific transformation texture and finally affects the mechanical properties of the alloys. The transformation lattice deformation associated selection mechanism has not been well addressed. In the
Although the genetic basis for endosperm devel-opment in maize (Zea mays) has been well studied, the mechanism for coordinating grain filling with increasing kernel size remains elusive. Here, we report that increased kernel size was selected during moder
Efficient genetic transformation has the potential to advance research and breeding in watermelon (Cit-rullus lanatus), but regeneration from tissue culture remains challenging. Previous work showed that expressing a fusion of two interacting transcriptio
Single crystal Ni-based superalloys are the typical structural materials for high-pressure turbine blades, and their microstructure is critical in determining their mechanical properties. The withdrawal rate is a key parameter affecting the microstructure
This research provides a new insight into designing next-generation filtration materials for Nano-pollutants removal from wastewater, and potentially for gas/solid or liquid/solid separation in corrosive environment. Considering the high corrosion and oxi
Grain size and leaf angle are key agronomic traits that determine final yields in rice. However, the underlying molecular mechanisms are not well un-derstood. Here we demonstrate that the Oryza sativa Mitogen Activated Protein Kinase Kinase Kinase OsMKKK7
目的 对牛痘疫苗接种家兔炎症皮肤提取物(神经妥乐平)治疗2型糖尿病(type 2 diabetes)同时合并周围神经病变(DPN)病人的作用效果和药物使用的安全性进行系统评价,为神经妥乐平在2型糖尿病病人同时合并DPN治疗中的合理使用提供循证证据.方法 检索循证图书馆、PubMed文献数据库、EMBASE、万方电子数据库、中国生物医学文献数据库(CBM)、中国期刊全文数据库(CNKI)等,按照筛选标准对神经妥乐平治疗DPN相关的临床随机对照研究(RCT)相关文章进行收集整理,检索时限从各数据库建库起至20
Traditional Chinese medicine (TCM) belongs to the most elaborate and extensive systems of plant-based healing. The herb Northern Ban Lan (Isatis tinctoria) is famous for its antiviral and anti-inflammatory activity. Although nu-merous components isolated
Primary dormant seeds of Arabidopsis thaliana did not germinate in darkness at temperature higher than 10–15°C. Ethylene improved the germination of dormant wild-type (Col-0) seeds at 25°C in darkness but seeds of the mutant affected in the proteolytic N-
Grain size is determined by the size and number of cells in the grain. The regulation of grain size is crucial for improving crop yield; however, the genes and molecular mechanisms that control grain size remain elusive. Here, we report that a member of t