【摘 要】
：
目的设计构建及表达纯化多房棘球绦虫葡萄糖转运蛋白1重组抗原肽(GLEP),为后续疫苗研究寻找有效工具。方法用生物信息学软件预测EmGLUT1跨膜结构,将设计构建的表达载体pCzn1-
【基金项目】
：
青海省科技厅自然科学基金青年项目(2020-ZJ-963Q),国家自然科学基金项目(81860299)。
论文部分内容阅读
目的设计构建及表达纯化多房棘球绦虫葡萄糖转运蛋白1重组抗原肽(GLEP),为后续疫苗研究寻找有效工具。方法用生物信息学软件预测EmGLUT1跨膜结构,将设计构建的表达载体pCzn1-GLEP转化入大肠杆菌真核表达系统,经IPTG诱导、Ni-NTA纯化获得目的蛋白。结果生物信息学软件分析结果表明EmGLUT1为12次跨膜蛋白,用柔性序列GS和KK将筛选后的肽段进行串联,构建重组表达载体pCzn1-GLEP,测序及酶切验证成功后,转化入Artic express大肠杆菌表达系统(表达部位鉴定属于可溶性表达),
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