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目的探讨刺参糖胺聚糖对单纯疱疹病毒(herpes simplex virus,HSV)Ⅰ型的抑制作用以及对感染小鼠的保护作用。方法以Vero细胞为靶细胞,接种HSV-1SM44毒株,同时分别加入不同浓度的刺参糖胺聚糖,观察细胞病变和定量PCR检测刺参糖胺聚糖的抗病毒作用;体内实验以HSV-1SM44毒株颅内接种感染小鼠,4h后以不同剂量的刺参糖胺聚糖灌胃,观察刺参糖胺聚糖对感染病毒小鼠的保护作用。结果刺参糖胺聚糖浓度在1.6mg.mL-1以上时,表现出对Vero细胞的毒性作用;在0.25~0.2mg.mL-1浓度范围表现出抗病毒活性,且具有一定的量效关系,对细胞无毒性作用。动物实验表明颅内感染小鼠刺参糖胺聚糖灌胃后,存活时间延长,死亡数明显减少;且呈一定正相关。结论刺参糖胺聚糖具有抗HSV-Ⅰ、保护HSV-Ⅰ感染小鼠的作用。
Objective To investigate the inhibitory effect of glycosaminoglycan from sea cucumber on herpes simplex virus (HSV) type I and its protective effect on infected mice. Methods Vero cells were used as target cells to inoculate HSV-1 SM44 strain, and different concentrations of glycosaminoglycans were added at the same time to observe cytopathic effect and quantitative PCR detection of antiviral effect of glycosaminoglycans of sea cucumber. HSV was used in vivo experiment. -1SM44 strain was intracranially inoculated to infected mice. Four hours later, different doses of glycosaminoglycans were administered orally to observe the protective effect of glycosaminoglycans from sea cucumber on infected mice. Results When the concentration of glycosaminoglycan in sea cucumber was above 1.6mg.mL-1, it showed toxic effect on Vero cells; it showed antiviral activity in the concentration range of 0.25~0.2mg.mL-1, and had a certain dose effect. Relationship, no toxicity to cells. Animal experiments showed that after intragastric inoculation of mice with glycosaminoglycans, the survival time was prolonged and the number of deaths was significantly reduced; there was a positive correlation. Conclusion The glycosaminoglycans of sea cucumber have the effect of resisting HSV-I and protecting mice infected with HSV-I.