论文部分内容阅读
目的:探讨姜黄素(curcumin,Cur)对胃癌细胞系HGC-27细胞增殖及侵袭力的影响以阐明Cur的抗瘤机制。方法:采用MTT法检测不同剂量(0、10、20、30、40?mol/L)及不同处理时间(24、48、72h)的姜黄素对HGC-27细胞增殖活性的影响;Transwell侵袭实验检测姜黄素对HGC-27细胞侵袭力的抑制作用。结果:1.MTT法显示姜黄素显著抑制HGC-27细胞增殖,20μmol/L,30μmol/L,40μmol/L姜黄素处理72小时其生长抑制率分别为24.63%,32.42%,76.43%,有明显的剂量及时间依赖性,不同剂量组之间及不同处理时间组之间比较有差异显著性(P<0.05)。2.Transwell侵袭实验显示姜黄素显著抑制HGC-27细胞的侵袭力,呈剂量和时间依赖性,各剂量组与对照组比较差异有显著性(P<0.05)。结论:姜黄素通过抑制HGC-27细胞增殖和侵袭行为起到抗肿瘤作用。
Objective: To investigate the effect of curcumin (Cur) on the proliferation and invasiveness of gastric cancer cell line HGC-27 in order to clarify the anti-tumor mechanism of Cur. Methods: The effect of curcumin on the proliferation of HGC-27 cells was detected by MTT assay at different dose (0, 10, 20, 30, 40 μmol / L) and different treatment time (24, 48, 72 h) Inhibitory effect of curcumin on invasiveness of HGC-27 cells. The results of MTT assay showed that curcumin significantly inhibited the proliferation of HGC-27 cells. The growth inhibition rates of curcumin treated with 20μmol / L, 30μmol / L and 40μmol / L curcumin were 24.63%, 32.42% and 76.43% Dose and time dependent. There was significant difference between different dose groups and different treatment time groups (P <0.05). Transwell invasion assay showed that curcumin significantly inhibited the invasiveness of HGC-27 cells in a dose and time-dependent manner, and there was a significant difference between each dose group and the control group (P <0.05). Conclusion: Curcumin has anti-tumor effect by inhibiting the proliferation and invasion of HGC-27 cells.