论文部分内容阅读
目的:探讨还原型谷胱甘肽(GSH)对毒鼠强(TET)中毒大鼠心脑的保护作用。方法:健康SD大鼠30只,随机分为假手术组、对照组、GSH预处理组,每组10只。通过TET灌胃染毒大鼠制备动物实验模型,染毒前30 min分别给予对照组、GSH预处理组腹腔注射生理盐水及GSH(1 000 mg/kg),观察各组大鼠的心脑组织病理学改变,免疫组织化学方法评价脑组织γ-氨基丁酸(GABA)、神经元特异性烯醇化酶(NSE)的表达水平。结果:GSH能明显延长TET染毒大鼠的惊厥潜伏期、缓解中毒症状、降低中毒大鼠的病死率,增加脑组织GABA的水平、减少NSE释放,减轻染毒大鼠心脑组织损伤。结论:GSH对TET中毒大鼠有保护作用,为进一步的临床使用提供理论依据。
Objective: To investigate the protective effect of reduced glutathione (GSH) on the heart and brain of tetramine poisoning rats. Methods: Thirty healthy SD rats were randomly divided into sham operation group, control group and GSH pretreatment group, 10 rats in each group. Animal model was established by intragastric administration of TET. The rats were given the control group 30 minutes before exposure. The GSH pretreatment group was injected with normal saline and GSH (1 000 mg / kg) intraperitoneally to observe the changes of heart and brain tissue The pathological changes and immunohistochemistry were used to evaluate the expression of γ-aminobutyric acid (GABA) and neuron-specific enolase (NSE) in brain tissue. Results: GSH could significantly prolong the latent period of convulsion in TET-treated rats, alleviate the symptoms of poisoning, reduce the mortality of poisoned rats, increase the level of GABA in brain tissue, decrease the release of NSE, and alleviate the injury of heart and brain in exposed rats. Conclusion: GSH has a protective effect on rats with TET poisoning, providing a theoretical basis for further clinical use.