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目的探讨自动摄影术用于观察常规尼龙绢集卵毛蚴孵化实验结果的价值。方法在牛粪便中掺入一定量的血吸虫新鲜虫卵,以含虫卵的粪便为实验组,包括低污染组和高污染组;以不含虫卵的粪便为阴性对照组,每组40份样本。以常规血吸虫尼龙绢集卵孵化法对粪便样本进行毛蚴孵化,采用手电筒光照、放大镜及自动录像相结合的方法,对粪便样本中毛蚴孵化的过程进行录像。通过观察录像记录,判断粪便样本中是否有血吸虫毛蚴孵出。同时采用常规肉眼观察方法进行结果判断,比较自动摄影术与肉眼观察法2种方法的毛蚴阳性检出率差异及优缺点。结果建立了手电筒光照、放大镜影像放大及自动摄影术相结合的毛蚴孵化结果观察方法,证实了自动摄影术对粪便中血吸虫虫卵毛蚴孵化的阳性检出率高于常规肉眼观察方法,尤其是在虫卵低污染组检出率有明显的差异。40份低污染组中常规肉眼观察法和自动摄影术观察法的阳性检出率分别为57.5%和85.0%(χ~2=11.723,P<0.05),40份高污染组中阳性检出率分别为97.5%和100%(χ~2=1.253,P>0.05),80份高低污染组平均阳性检出率分别为77.5%和92.5%(χ~2=6.894,P<0.05)。结论自动摄影术可有效提高毛蚴常规尼龙绢集卵孵化实验的阳性检出率。
Objective To investigate the value of automatic photogrammetry in observing the experimental results of hatching experiment of sericolysis of routine nylon filament yarn. Methods A new amount of eggs of Schistosoma japonicum was mixed into cow manure, and the excrement containing eggs was used as experimental group, including low-pollution group and high-pollution group. The negative control group without egg was used as control group, 40 sample. The hatching of miracidia was carried out on the stool samples by routine silkworm eggs hatching method. The hatching process of miracidia in the stool samples was recorded by the combination of flashlight illumination, magnifying glass and automatic recording. By observing the video recording to determine whether there is stool samples schistosome miracidia hatch. At the same time, the results of routine macroscopic observation were used to judge the results. The difference of the positive detection rate and the advantages and disadvantages between the two methods of automatic photography and macroscopic observation were compared. Results The establishment of a method of observation of the hatching of the miracidia by flashlight illumination, magnifying glass magnification and automatic photography confirmed the positive detection rate of autoimmunity on the incubation of schistosome eggs in the stool by routine photomicrographs, especially in the Detection rate of low egg contamination group has obvious difference. The positive detection rates of routine macroscopic observation and automatic photography observation in 40 low-contamination groups were 57.5% and 85.0% (χ ~ 2 = 11.723, P <0.05) respectively. The positive detection rate in 40 high-pollution groups (Χ ~ 2 = 1.253, P> 0.05). The average positive detection rates of the 80 high and low pollution groups were 77.5% and 92.5% (χ ~ 2 = 6.894, P <0.05). Conclusion Automatic photogrammetry can effectively improve the positive detection rate of hatching experiment of filamentous silkworm eggs accumulated in conventional miRNAs.