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基于弱酸介质中,在微乳液介质存在时,痕量硒(IV)催化KBrO3氧化2-(5-硝苯2-吡啶偶氮)-5-二已氨基酚(5-NO2-PADAP)的褪色反应,建立了测定痕量硒(IV)的动力学光度法。在固定加热时间段(8min)后,于450nm处测定5-NO2-PADAP的吸光度降低值监控反应速率。方法检出限为0.013μg/L,校准曲线的浓度线性范围为0.0～1.0μg/L。结合巯基葡聚糖凝胶分离富集,方法用于食物和人发样品中痕量硒(IV)的测定,结果与ICP-AES测定值相符,相对标准偏差为2.9%～4.3%,加标回收率为96.3%～103.8%。
In the presence of microemulsion medium, trace amounts of selenium (IV) catalyze the fading of 2- (5-nitrophenyl 2-pyridylazo) -5-diaminophenol (5-NO2-PADAP) Reaction, established a kinetic spectrophotometric method for the determination of trace selenium (IV). The reduction in absorbance of 5-NO2-PADAP was measured at 450 nm after a fixed heating period (8 min) to monitor the reaction rate. The detection limit of this method was 0.013 μg / L. The linear range of the calibration curve was 0.0 ~ 1.0 μg / L. The method was applied to the determination of trace selenium (IV) in food and human hair samples. The results were in good agreement with the results of ICP-AES. The relative standard deviations (RSDs) were 2.9% -4.3% The recovery rate was 96.3% ~ 103.8%.