论文部分内容阅读
目的观察小鼠感染裂头蚴后外周血T淋巴细胞亚群及细胞因子含量的动态变化,了解小鼠感染后的细胞免疫特点。方法从黑斑蛙体内检获裂头蚴,口服法感染昆明小鼠,5条/只。感染后第2～10周分批处死小鼠,用流式细胞仪检测外周血T淋巴细胞亚群(CD4+T、CD8+T),用双抗夹心ELISA法检测血清IFN-γ、TNF-α、IL-4、IL-10的含量变化。结果实验鼠CD4+T细胞百分比在感染后第2～5周呈上升趋势,之后逐渐下降,第9周降至(31.61±5.33)%,第10周又回升到第8周水平;CD8+T细胞百分比升高,第4周达高峰,为(41.69±15.84)%。IFN-γ在感染后第3周为(13.36±2.58)pg/ml,第4周为(13.96±4.53)pg/ml,第5周为(13.33±2.32)pg/ml,与对照组相比差异有统计学意义(P<0.05);TNF-α第4周达(22.99±4.52)pg/ml,之后呈下降趋势,第10周降至(10.99±1.14)pg/ml;IL-4第4周开始上升,第7周达(18.31±7.70)pg/ml,第8周后逐渐下降;IL-10从第5周起逐渐上升,至第8周达到(15.89±4.64)pg/ml,之后呈下降趋势。结论小鼠感染裂头蚴早期,发生Th1免疫应答;随着感染的进程,免疫应答向Th2偏移。Th1/Th2型免疫应答发生的时相和效应强度可能影响裂头蚴感染的最终结局。
Objective To observe the dynamic changes of T lymphocyte subsets and cytokines in peripheral blood of mice infected with schizophrenia, and to understand the characteristics of cellular immunity after mouse infection. Methods The schistosomes were harvested from black spot frogs and infected with Kunming mice by oral administration. Mice were sacrificed in batches from 2 to 10 weeks after infection, peripheral blood T lymphocyte subsets (CD4 + T, CD8 + T) were detected by flow cytometry, serum IFN-γ, TNF- α, IL-4, IL-10 content changes. Results The percentages of CD4 + T cells in the experimental group increased from the second week to the fifth week after infection, and then decreased gradually. The percentage of CD4 + T cells decreased to (31.61 ± 5.33)% in the ninth week and returned to the eighth week in the tenth week. CD8 + T The percentage of cells increased and peaked at the fourth week, which was (41.69 ± 15.84)%. IFN-γ was (13.36 ± 2.58) pg / ml in the third week after infection, (13.96 ± 4.53) pg / ml in the fourth week and (13.33 ± 2.32) pg / ml in the fifth week, The difference was statistically significant (P <0.05). The level of TNF-α was (22.99 ± 4.52) pg / ml in the fourth week, then decreased and decreased to (10.99 ± 1.14) pg / ml in the tenth week. The level of IL-10 gradually increased from the fifth week to the fifth week (15.89 ± 4.64) pg / ml, the third week was (18.31 ± 7.70) pg / ml, After a downward trend. Conclusion The mice infected with the early stage of the larvae have a Th1 immune response. As the infection progresses, the immune response shifts to Th2. The timing and magnitude of the effects of Th1 / Th2 immune responses may affect the final outcome of the infection with the splenocyte.